Supersensitive gastrin assay using antibodies raised against a cholecystokinin homolog

Research output: Contribution to journalArticle

Abstract

Peptide hormones may occur in particularly low amounts in samples from small animals. Hence, in a rat microdialysis study conventional immunoassays were not sufficiently sensitive to measure gastrin in the dialysis samples. We therefore exploited the observation that antibodies raised against the homologous hormone cholecystokinin (CCK) occasionally bind gastrin peptides with significantly higher affinity than the proper ligand. The immunoassay thus established could detect 1.0 pmol/l in 15 mu l microdialysate, which corresponds to 23 attomol gastrin. Such detection limit is five-fold lower than that obtained with the most avid conventional gastrin antibodies. The results may encourage similar approaches for other peptides using homologue-raised antibodies when supersensitivity is required.

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Authors
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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Endocrinology and Diabetes

Keywords

  • peptide hormones, radioimmunoassay (RIA), homology, cholecystokinin (CCK), gastrin, Antibody cooperativity
Original languageEnglish
Pages (from-to)175-179
JournalScandinavian Journal of Clinical & Laboratory Investigation
Volume72
Issue number2
Publication statusPublished - 2012
Publication categoryResearch
Peer-reviewedYes