Supersensitive gastrin assay using antibodies raised against a cholecystokinin homolog

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Supersensitive gastrin assay using antibodies raised against a cholecystokinin homolog. / Rehfeld, Jens F.; Ericsson, Peter.

In: Scandinavian Journal of Clinical & Laboratory Investigation, Vol. 72, No. 2, 2012, p. 175-179.

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TY - JOUR

T1 - Supersensitive gastrin assay using antibodies raised against a cholecystokinin homolog

AU - Rehfeld, Jens F.

AU - Ericsson, Peter

PY - 2012

Y1 - 2012

N2 - Peptide hormones may occur in particularly low amounts in samples from small animals. Hence, in a rat microdialysis study conventional immunoassays were not sufficiently sensitive to measure gastrin in the dialysis samples. We therefore exploited the observation that antibodies raised against the homologous hormone cholecystokinin (CCK) occasionally bind gastrin peptides with significantly higher affinity than the proper ligand. The immunoassay thus established could detect 1.0 pmol/l in 15 mu l microdialysate, which corresponds to 23 attomol gastrin. Such detection limit is five-fold lower than that obtained with the most avid conventional gastrin antibodies. The results may encourage similar approaches for other peptides using homologue-raised antibodies when supersensitivity is required.

AB - Peptide hormones may occur in particularly low amounts in samples from small animals. Hence, in a rat microdialysis study conventional immunoassays were not sufficiently sensitive to measure gastrin in the dialysis samples. We therefore exploited the observation that antibodies raised against the homologous hormone cholecystokinin (CCK) occasionally bind gastrin peptides with significantly higher affinity than the proper ligand. The immunoassay thus established could detect 1.0 pmol/l in 15 mu l microdialysate, which corresponds to 23 attomol gastrin. Such detection limit is five-fold lower than that obtained with the most avid conventional gastrin antibodies. The results may encourage similar approaches for other peptides using homologue-raised antibodies when supersensitivity is required.

KW - peptide hormones

KW - radioimmunoassay (RIA)

KW - homology

KW - cholecystokinin (CCK)

KW - gastrin

KW - Antibody cooperativity

U2 - 10.3109/00365513.2011.637130

DO - 10.3109/00365513.2011.637130

M3 - Article

VL - 72

SP - 175

EP - 179

JO - Scandinavian Journal of Clinical & Laboratory Investigation

T2 - Scandinavian Journal of Clinical & Laboratory Investigation

JF - Scandinavian Journal of Clinical & Laboratory Investigation

SN - 1502-7686

IS - 2

ER -