Suppression of external NADPH dehydrogenase—NDB1 in arabidopsis thaliana confers improved tolerance to ammonium toxicity via efficient Glutathione/Redox metabolism

Research output: Contribution to journalArticle

Abstract

Environmental stresses, including ammonium (NH4 +) nourishment, can damage key mitochondrial components through the production of surplus reactive oxygen species (ROS) in the mitochondrial electron transport chain. However, alternative electron pathways are significant for efficient reductant dissipation in mitochondria during ammonium nutrition. The aim of this study was to define the role of external NADPH-dehydrogenase (NDB1) during oxidative metabolism of NH4 +-fed plants. Most plant species grown with NH4 + as the sole nitrogen source experience a condition known as “ammonium toxicity syndrome”. Surprisingly, transgenic Arabidopsis thaliana plants suppressing NDB1 were more resistant to NH4 + treatment. The NDB1 knock-down line was characterized by milder oxidative stress symptoms in plant tissues when supplied with NH4 +. Mitochondrial ROS accumulation, in particular, was attenuated in the NDB1 knock-down plants during NH4 + treatment. Enhanced antioxidant defense, primarily concerning the glutathione pool, may prevent ROS accumulation in NH4 +-grown NDB1-suppressing plants. We found that induction of glutathione peroxidase-like enzymes and peroxiredoxins in the NDB1-surpressing line contributed to lower ammonium-toxicity stress. The major conclusion of this study was that NDB1 suppression in plants confers tolerance to changes in redox homeostasis that occur in response to prolonged ammonium nutrition, causing cross tolerance among plants.

Details

Authors
  • Anna Podgórska
  • Monika Ostaszewska-Bugajska
  • Klaudia Borysiuk
  • Agata Tarnowska
  • Monika Jakubiak
  • Maria Burian
  • Allan G. Rasmusson
  • Bożena Szal
Organisations
External organisations
  • University of Warsaw
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell Biology
  • Botany

Keywords

  • Ammonium toxicity, External type II NADPH dehydrogenase, Glutathione metabolism, Reactive oxygen species, Redox homeostasis
Original languageEnglish
Article number1412
JournalInternational Journal of Molecular Sciences
Volume19
Issue number5
Publication statusPublished - 2018 May 9
Publication categoryResearch
Peer-reviewedYes