Supramolecular Organization in Self-Assembly of Chromatin and Cationic Lipid Bilayers is Controlled by Membrane Charge Density

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Supramolecular Organization in Self-Assembly of Chromatin and Cationic Lipid Bilayers is Controlled by Membrane Charge Density. / Berezhnoy, Nikolay V.; Lundberg, Dan; Korolev, Nikolay; Lu, Chenning; Yan, Jiang; Miguel, Maria; Lindman, Björn; Nordenskioeld, Lars.

In: Biomacromolecules, Vol. 13, No. 12, 2012, p. 4146-4157.

Research output: Contribution to journalArticle

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Berezhnoy, NV, Lundberg, D, Korolev, N, Lu, C, Yan, J, Miguel, M, Lindman, B & Nordenskioeld, L 2012, 'Supramolecular Organization in Self-Assembly of Chromatin and Cationic Lipid Bilayers is Controlled by Membrane Charge Density', Biomacromolecules, vol. 13, no. 12, pp. 4146-4157. https://doi.org/10.1021/bm301436x

APA

Berezhnoy, N. V., Lundberg, D., Korolev, N., Lu, C., Yan, J., Miguel, M., ... Nordenskioeld, L. (2012). Supramolecular Organization in Self-Assembly of Chromatin and Cationic Lipid Bilayers is Controlled by Membrane Charge Density. Biomacromolecules, 13(12), 4146-4157. https://doi.org/10.1021/bm301436x

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Berezhnoy, Nikolay V. ; Lundberg, Dan ; Korolev, Nikolay ; Lu, Chenning ; Yan, Jiang ; Miguel, Maria ; Lindman, Björn ; Nordenskioeld, Lars. / Supramolecular Organization in Self-Assembly of Chromatin and Cationic Lipid Bilayers is Controlled by Membrane Charge Density. In: Biomacromolecules. 2012 ; Vol. 13, No. 12. pp. 4146-4157.

RIS

TY - JOUR

T1 - Supramolecular Organization in Self-Assembly of Chromatin and Cationic Lipid Bilayers is Controlled by Membrane Charge Density

AU - Berezhnoy, Nikolay V.

AU - Lundberg, Dan

AU - Korolev, Nikolay

AU - Lu, Chenning

AU - Yan, Jiang

AU - Miguel, Maria

AU - Lindman, Björn

AU - Nordenskioeld, Lars

PY - 2012

Y1 - 2012

N2 - In this work we have investigated the structures of aggregates formed in model systems of dilute aqueous mixtures of "model chromatin" consisting of either recombinant nucleosome core particles (NCPs) or nucleosome arrays consisting of 12 NCPs connected with 30 bp linker DNA, and liposomes made from different mixtures of cationic and zwitterionic lipids, 1,2-dioleoyl-3-trimethylammonium-propane chloride salt (DOTAP) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). The aggregates formed were characterized using different optical microscopy methods and small-angle X-ray scattering (SAXS), and the results are discussed in terms of the competing intermolecular interactions among the components. For a majority of the samples, the presence of lamellar structures could be identified. Ir. samples with high fractions of DOTAP in the liposomes, well-defined lamellar structures very similar to those formed by the corresponding lipid mixtures and DNA alone (i.e., without histone proteins) were observed; in these aggregates, the histones are expelled from the model chromatin. The findings suggest that, with liposomes containing large fractions of cationic lipid, the dominating driving force for aggregation is the increase in translational entropy from the release of counterions, whereas with lower fractions of the cationic lipid, the entropy of mixing of the lipids within the bilayers results in a decreased DNA-lipid attraction.

AB - In this work we have investigated the structures of aggregates formed in model systems of dilute aqueous mixtures of "model chromatin" consisting of either recombinant nucleosome core particles (NCPs) or nucleosome arrays consisting of 12 NCPs connected with 30 bp linker DNA, and liposomes made from different mixtures of cationic and zwitterionic lipids, 1,2-dioleoyl-3-trimethylammonium-propane chloride salt (DOTAP) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). The aggregates formed were characterized using different optical microscopy methods and small-angle X-ray scattering (SAXS), and the results are discussed in terms of the competing intermolecular interactions among the components. For a majority of the samples, the presence of lamellar structures could be identified. Ir. samples with high fractions of DOTAP in the liposomes, well-defined lamellar structures very similar to those formed by the corresponding lipid mixtures and DNA alone (i.e., without histone proteins) were observed; in these aggregates, the histones are expelled from the model chromatin. The findings suggest that, with liposomes containing large fractions of cationic lipid, the dominating driving force for aggregation is the increase in translational entropy from the release of counterions, whereas with lower fractions of the cationic lipid, the entropy of mixing of the lipids within the bilayers results in a decreased DNA-lipid attraction.

U2 - 10.1021/bm301436x

DO - 10.1021/bm301436x

M3 - Article

VL - 13

SP - 4146

EP - 4157

JO - Biomacromolecules

T2 - Biomacromolecules

JF - Biomacromolecules

SN - 1526-4602

IS - 12

ER -