Surface characterization of the Gram-positive bacteria Bacillus subtilis - an XPS study

Research output: Contribution to journalArticle

Abstract

Interactions at the bacteria-solid interface in aquatic environments are of crucial importance in biofilm formation processes. The characterization of microbial surfaces properties is therefore a very important step for the understanding of these interactions. In this study, XPS spectra of Bacillus subtilis wet pastes centrifuged from suspensions over a span of pH values were recorded using a fast-freezing procedure. The chemical composition of the bacterial surface was elucidated and the presence of surface sites containing carboxylate, phosphate and amine functional groups was established. It was possible to distinguish between charged and uncharged amine sites on the surface and follow their deprotonation reactions as a function of pH. It is suggested that increased metabolic activity around the physiological pH results in the excretion of proteins and/or peptides which are possibly related to the production of extracellular polymeric substances (EPS). Copyright (C) 2006 John Wiley & Sons, Ltd.

Details

Authors
External organisations
  • External Organization - Unknown
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Earth and Related Environmental Sciences
Original languageEnglish
Pages (from-to)202-205
JournalSurface and Interface Analysis
Volume38
Publication statusPublished - 2006
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes

Bibliographic note

4