Surface Microdialysis Detects Ischemia After Esophageal Resection—An Experimental Animal Study

Research output: Contribution to journalArticle

Abstract

Background: After an esophageal resection, continuity is commonly restored by a gastric tube reconstruction and an intrathoracic anastomosis to the remaining proximal esophagus. Ischemia of the anastomotic region is considered to play a pivotal role in anastomotic leakage. Microdialysis (μD) is an excellent method to measure local biochemical substances and parameters in a specific organ or compartment aiming at early detection of ischemia. This animal study evaluates ischemia of the gastric tube reconstruction using a novel method—μD on organ surfaces. This promising method may have the potential to detect an anastomotic leakage before clinical symptoms develop. Methods: Anesthetized normoventilated pigs were used. Surface microdialysis (S-μD) catheters and an intraparenchymal oxygen tension catheter were placed on the stomach. A gastric tube was made and the gastroepiploic artery was divided halfway along the greater curvature to produce severe ischemia at the top of the gastric tube. μD data from four locations (gastric tube, ileum and peritoneal cavity) were recorded every 20 min during the experiment. Tissue samples from all catheter sites underwent histopathological analysis. Intraparenchymal oxygen partial pressure, systemic blood tests, and hemodynamic parameters were recorded. Results: S-μD data showed values indicating severe ischemia at the top of the gastric tube and intermediate ischemia at the level of transection of the gastroepiploic artery. Ischemia was verified by histopathological analysis of tissue samples and intraparenchymal oxygen tension data. Conclusions: S-μD can detect and grade severity of local ischemia in real time, in an animal model.

Details

Authors
Organisations
External organisations
  • Skåne University Hospital
  • Umeå University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Surgery
  • Gastroenterology and Hepatology

Keywords

  • Ischemia, Metabolism, Microdialysis
Original languageEnglish
Pages (from-to)537-543
Number of pages7
JournalJournal of Surgical Research
Volume245
Publication statusPublished - 2020
Publication categoryResearch
Peer-reviewedYes