Survival and functional recovery of transplanted human dopaminergic neurons into hemiparkinsonian rats depend on the cannula size of the implantation instrument

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Survival and functional recovery of transplanted human dopaminergic neurons into hemiparkinsonian rats depend on the cannula size of the implantation instrument. / Steiner, Barbara; Winter, Christine; Blumensath, Stefan; Paul, Gesine; Harnack, Daniel; Nikkhah, Guido; Kupsch, Andreas.

In: Journal of Neuroscience Methods, Vol. 169, No. 1, 30.03.2008, p. 128-134.

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Steiner, Barbara ; Winter, Christine ; Blumensath, Stefan ; Paul, Gesine ; Harnack, Daniel ; Nikkhah, Guido ; Kupsch, Andreas. / Survival and functional recovery of transplanted human dopaminergic neurons into hemiparkinsonian rats depend on the cannula size of the implantation instrument. In: Journal of Neuroscience Methods. 2008 ; Vol. 169, No. 1. pp. 128-134.

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TY - JOUR

T1 - Survival and functional recovery of transplanted human dopaminergic neurons into hemiparkinsonian rats depend on the cannula size of the implantation instrument

AU - Steiner, Barbara

AU - Winter, Christine

AU - Blumensath, Stefan

AU - Paul, Gesine

AU - Harnack, Daniel

AU - Nikkhah, Guido

AU - Kupsch, Andreas

PY - 2008/3/30

Y1 - 2008/3/30

N2 - Promising therapeutic strategies for neurodegenerative diseases such as Parkinson's disease include replacement of lost striatal dopaminergic neurons by grafting of embryonic mesencephalic cells. However, the poor survival of the transplanted tissue still limits transplantation of these cells into the human brain in a larger number of patients. We addressed the question, if the diameter of the transplantation cannulas has an effect on the number of surviving transplanted human embryonic mesencephalic cells into the striatum of 6-OHDA lesioned rats. We report a significantly higher number of surviving human cells using an ultrathin micropipette compared to cannulas with wider diameters. Importantly, higher numbers of surviving cells also correlated with a behavioral recovery of the hemiparkinsonian rats.

AB - Promising therapeutic strategies for neurodegenerative diseases such as Parkinson's disease include replacement of lost striatal dopaminergic neurons by grafting of embryonic mesencephalic cells. However, the poor survival of the transplanted tissue still limits transplantation of these cells into the human brain in a larger number of patients. We addressed the question, if the diameter of the transplantation cannulas has an effect on the number of surviving transplanted human embryonic mesencephalic cells into the striatum of 6-OHDA lesioned rats. We report a significantly higher number of surviving human cells using an ultrathin micropipette compared to cannulas with wider diameters. Importantly, higher numbers of surviving cells also correlated with a behavioral recovery of the hemiparkinsonian rats.

KW - Cannula size

KW - Human dopaminergic cells

KW - Transplantation

UR - http://www.scopus.com/inward/record.url?scp=39749112525&partnerID=8YFLogxK

U2 - 10.1016/j.jneumeth.2007.11.032

DO - 10.1016/j.jneumeth.2007.11.032

M3 - Article

C2 - 18241930

AN - SCOPUS:39749112525

VL - 169

SP - 128

EP - 134

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 1872-678X

IS - 1

ER -