Synthesis and use of protein G imprinted cryogel as affinity matrix to purify protein G from cell lyaste.

Research output: Contribution to journalArticle

Abstract

Monolithic macroporous cryogel imprinted with protein G was prepared using a functional co-monomer of N-methacryloyl-l-phenylalanine and 2-hydroxyethyl methacrylate. The chemical structure of the cryogel prepared was studied by FTIR-spectroscopy and its porosity was analysed using scanning electron microscopy. The cryogel was used to purify protein G from recombinant Escherichia coli cell lysate and the effect of pH, temperature, ionic strength, flow rate, etc on the adsorption of protein G to the monolithic column have been investigated. The selectivity of the imprinted cryogel was studied using protein A and myoglobin. It was possible to capture about 9mg of Protein G per g of the cryogel.

Details

Authors
Organisations
External organisations
  • Hacettepe University
  • Indienz AB
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Structural Biology
Original languageEnglish
Pages (from-to)204-212
JournalJournal of Chromatography. B
Volume1021
Early online date2016 Jan 4
Publication statusPublished - 2016
Publication categoryResearch
Peer-reviewedYes