Systematic Identification of Single Amino Acid Variants in Glioma Stem-Cell-Derived Chromosome 19 Proteins

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Systematic Identification of Single Amino Acid Variants in Glioma Stem-Cell-Derived Chromosome 19 Proteins. / Lichti, Cheryl; Mostovenko, Ekaterina; Wadsworth, Paul; Lynch, Gillian; Pettitt, Montgomery; Sulman, Erik; Wang, Qiunghu; Lang, Frederick; Rezeli, Melinda; Marko-Varga, György; Végvári, Ákos; Nilsson, Carol.

In: Journal of Proteome Research, Vol. 14, No. 2, 2015, p. 778-786.

Research output: Contribution to journalArticle

Harvard

Lichti, C, Mostovenko, E, Wadsworth, P, Lynch, G, Pettitt, M, Sulman, E, Wang, Q, Lang, F, Rezeli, M, Marko-Varga, G, Végvári, Á & Nilsson, C 2015, 'Systematic Identification of Single Amino Acid Variants in Glioma Stem-Cell-Derived Chromosome 19 Proteins', Journal of Proteome Research, vol. 14, no. 2, pp. 778-786. https://doi.org/10.1021/pr500810g

APA

Lichti, C., Mostovenko, E., Wadsworth, P., Lynch, G., Pettitt, M., Sulman, E., ... Nilsson, C. (2015). Systematic Identification of Single Amino Acid Variants in Glioma Stem-Cell-Derived Chromosome 19 Proteins. Journal of Proteome Research, 14(2), 778-786. https://doi.org/10.1021/pr500810g

CBE

Lichti C, Mostovenko E, Wadsworth P, Lynch G, Pettitt M, Sulman E, Wang Q, Lang F, Rezeli M, Marko-Varga G, Végvári Á, Nilsson C. 2015. Systematic Identification of Single Amino Acid Variants in Glioma Stem-Cell-Derived Chromosome 19 Proteins. Journal of Proteome Research. 14(2):778-786. https://doi.org/10.1021/pr500810g

MLA

Vancouver

Author

Lichti, Cheryl ; Mostovenko, Ekaterina ; Wadsworth, Paul ; Lynch, Gillian ; Pettitt, Montgomery ; Sulman, Erik ; Wang, Qiunghu ; Lang, Frederick ; Rezeli, Melinda ; Marko-Varga, György ; Végvári, Ákos ; Nilsson, Carol. / Systematic Identification of Single Amino Acid Variants in Glioma Stem-Cell-Derived Chromosome 19 Proteins. In: Journal of Proteome Research. 2015 ; Vol. 14, No. 2. pp. 778-786.

RIS

TY - JOUR

T1 - Systematic Identification of Single Amino Acid Variants in Glioma Stem-Cell-Derived Chromosome 19 Proteins

AU - Lichti, Cheryl

AU - Mostovenko, Ekaterina

AU - Wadsworth, Paul

AU - Lynch, Gillian

AU - Pettitt, Montgomery

AU - Sulman, Erik

AU - Wang, Qiunghu

AU - Lang, Frederick

AU - Rezeli, Melinda

AU - Marko-Varga, György

AU - Végvári, Ákos

AU - Nilsson, Carol

PY - 2015

Y1 - 2015

N2 - Novel proteoforms with single amino acid polymorphism represent proteins that often have altered biological functions but are less explored in the human proteome. We have developed an approach, searching high quality shotgun proteomic data against an extended protein database, to identify expressed mutant proteoforms in glioma stem cell (GSC) lines. The systematic search of MS/MS spectra has recognized 13 chromosome 19 proteins in GSCs with altered amino acid sequences using PEAKS 7.0 as the search engine. The results were further verified by manual spectral examination, validating 14 proteoforms. One of the novel findings, a mutant form of branched-chain aminoacyl transferase 2 (T186R), was verified at the transcript level and by SRM in several glioma stem cell lines. The structure of this proteoform examined by molecular modeling to estimate structural changes of mutation that could lead to functional modifications potentially linked to glioma. Based on our initial findings, we believe that our approach presented could contribute to construct a more complete map of the human functional proteome.

AB - Novel proteoforms with single amino acid polymorphism represent proteins that often have altered biological functions but are less explored in the human proteome. We have developed an approach, searching high quality shotgun proteomic data against an extended protein database, to identify expressed mutant proteoforms in glioma stem cell (GSC) lines. The systematic search of MS/MS spectra has recognized 13 chromosome 19 proteins in GSCs with altered amino acid sequences using PEAKS 7.0 as the search engine. The results were further verified by manual spectral examination, validating 14 proteoforms. One of the novel findings, a mutant form of branched-chain aminoacyl transferase 2 (T186R), was verified at the transcript level and by SRM in several glioma stem cell lines. The structure of this proteoform examined by molecular modeling to estimate structural changes of mutation that could lead to functional modifications potentially linked to glioma. Based on our initial findings, we believe that our approach presented could contribute to construct a more complete map of the human functional proteome.

KW - Chromosome-centric Human Proteome Project

KW - Chromosome 19

KW - Mass spectrometry

KW - Non-synonymous single nucleotide polymorphism

KW - amino acid polymorphism

KW - Glioma stem cells

KW - Cancer proteomics

KW - bioinformatics

KW - SRM assay

KW - BCAT2 T186R

U2 - 10.1021/pr500810g

DO - 10.1021/pr500810g

M3 - Article

VL - 14

SP - 778

EP - 786

JO - Journal of Proteome Research

T2 - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 2

ER -