Systemic endotoxin administration results in increased S100B protein blood levels and periventricular brain white matter injury in the preterm fetal sheep

Research output: Contribution to journalArticle


Objective: Intrauterine infection is suggested to cause perinatal brain white matter injury. The aim of the present study was to clarify, whether intravenous application of endotoxin results in neuropathological findings and increased blood levels of the S100B protein, which is a consolidated marker of brain injury. Methods: Twenty-one fetal sheep were chronically catheterized at a mean gestational age of 107 +/- 1 days (0.7 of gestation). Three days after surgery fetuses received either 100 (n = 9), 500 (n = 5) or 2500 ng (n = 1) lipopolysaccharide (LPS; E. coli; O127:138, Sigma-Aldrich) or 2 ml 0.9% saline (n = 6) i.v. S100B protein blood levels were assessed before during and after LPS or placebo administration. Brain damage was evaluated by light microscopy. Selected areas of the periventricular white matter were also examined by electron microscopy. Results: Histopathological screening revealed no evidence for cortical neuronal cell damage in both groups. However, LPS treatment resulted in inflammatory infiltrates in all animals and cystic lesions in the periventricular brain white matter in two fetuses. On electron micrographs, infiltrate forming cells appeared to be activated microglia. S100B protein blood levels were significantly higher in the LPS group at 1 h (p < 0.01) after LPS injection, peaking at 3 h (p < 0.001) and returning to baseline between 12 and 72 h. Conclusion: Intravenous application of endotoxin caused focal periventricular brain white matter injury, inflammation and an increase in S100B protein release. It is suggested that longitudinal investigations of S100B protein blood levels offer a tool for the early detection of white matter injury. (c) 2005 Elsevier Ireland Ltd. All rights reserved.


  • Y Garnier
  • R Berger
  • S Alm
  • MU von Duering
  • ABC Coumans
  • F Michetti
  • Matteo Bruschettini
  • M Lituania
  • THM Hasaart
  • D Gazzolo
External organisations
  • External Organization - Unknown
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Obstetrics, Gynecology and Reproductive Medicine


  • leukomalacia, periventricular, circulation, brain injrury, response syndrom, fetal inflammatory, Escherichia coli, lipopolysaccharide, endotoxin
Original languageEnglish
Pages (from-to)15-22
JournalEuropean Journal of Obstetrics, Gynecology, and Reproductive Biology
Issue number1
Publication statusPublished - 2006
Publication categoryResearch
Externally publishedYes