T Follicular Helper, but Not Th1, Cell Differentiation in the Absence of Conventional Dendritic Cells.

Research output: Contribution to journalArticle

Abstract

Development of long-lived humoral immunity is dependent on CXCR5-expressing T follicular helper (Tfh) cells, which develop concomitantly to effector Th cells that support cellular immunity. Conventional dendritic cells (cDCs) are critical APCs for initial priming of naive CD4(+) T cells but, importantly, also provide accessory signals that govern effector Th cell commitment. To define the accessory role of cDCs during the concurrent development of Tfh and effector Th1 cells, we performed high-dose Ag immunization in conjunction with the Th1-biased adjuvant polyinosinic:polycytidylic acid (pI:C). In the absence of cDCs, pI:C failed to induce Th1 cell commitment and IgG2c production. However, cDC depletion did not impair Tfh cell differentiation or germinal center formation, and long-lived IgG1 responses of unaltered affinity developed in mice lacking cDCs at the time point for immunization. Thus, cDCs are required for the pI:C-driven Th1 cell fate commitment but have no crucial accessory function in relation to Tfh cell differentiation.

Details

Authors
  • Madelene Dahlgren
  • Tobias Gustafsson-Hedberg
  • Megan Livingston
  • Helena Cucak
  • Samuel Alsén
  • Ulf Yrlid
  • Bengt Johansson Lindbom
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area
Original languageEnglish
Pages (from-to)5187-5199
JournalJournal of Immunology
Volume194
Issue number11
Publication statusPublished - 2015
Publication categoryResearch
Peer-reviewedYes