Targeting the reactive intermediate in polysaccharide monooxygenases

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Abstract

Lytic polysaccharide monooxygenases (LPMOs) are copper metalloenzymes that can enhance polysaccharide depolymerization through an oxidative mechanism, making them interesting for the production of biofuel from cellulose. However, the details of this activation are unknown; in particular, the nature of the intermediate that attacks the glycoside C–H bond in the polysaccharide is not known, and a number of different species have been suggested. The homolytic bond-dissociation energy (BDE) has often been used as a descriptor for the bond-activation power, especially for inorganic model complexes. We have employed quantum-chemical cluster calculations to estimate the BDE for a number of possible LPMO intermediates to bridge the gap between model complexes and the actual LPMO active site. The calculated BDEs suggest that the reactive intermediate is either a Cu(II)–oxyl, a Cu(III)–oxyl, or a Cu(III)–hydroxide, which indicate that O–O bond breaking occurs before the C–H activation step.

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Subject classification (UKÄ) – MANDATORY

  • Chemical Sciences

Keywords

  • Lytic polysaccharide monooxygenase, Density functional theory, Reaction mechanism , Computational chemistry
Original languageEnglish
Number of pages9
JournalJournal of Biological Inorganic Chemistry
Publication statusPublished - 2017
Publication categoryResearch
Peer-reviewedYes