Tendon healing in vivo: An experimental model

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Tendon healing in vivo : An experimental model. / Abrahamsson, Sven Olof; Lundborg, G.; Lohmander, Stefan.

In: Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery, Vol. 23, No. 3, 1989, p. 199-205.

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TY - JOUR

T1 - Tendon healing in vivo

T2 - An experimental model

AU - Abrahamsson, Sven Olof

AU - Lundborg, G.

AU - Lohmander, Stefan

PY - 1989

Y1 - 1989

N2 - Flexor tendon segments were incubated in a diffusion chamber in the subcutis of rabbits. Tendons incubated up to 6 weeks in the diffusion chamber showed proliferating and migrating cells from the epitenon cell layer as well as viable endotenon cells. Explants frozen in liquid nitrogen prior to incubation showed no signs of extrinsic cell contamination and remained non-viable indicating that no cell penetration occurred through the Millipore filter and that cell division seen in non-frozen and incubated tendons was an expression of intrinsic cellular proliferative capacity of the tendon. In tendon segments incubated in chambers for three weeks, collagen synthesis was reduced by 50% and the rate of cell proliferation measured as 3H-thymidine incorporation, was 15 times that of native tendons. Frozen and incubated tendons showed only traces of remaining matrix synthesis or cell proliferation. With this experimental model we have histologically and biochemically shown that tendons may survive and heal while the nutrition exclusively could be based on diffusion and the tendons have an intrinsic capacity of healing. The described model enables further studies on tendon healing and its regulation.

AB - Flexor tendon segments were incubated in a diffusion chamber in the subcutis of rabbits. Tendons incubated up to 6 weeks in the diffusion chamber showed proliferating and migrating cells from the epitenon cell layer as well as viable endotenon cells. Explants frozen in liquid nitrogen prior to incubation showed no signs of extrinsic cell contamination and remained non-viable indicating that no cell penetration occurred through the Millipore filter and that cell division seen in non-frozen and incubated tendons was an expression of intrinsic cellular proliferative capacity of the tendon. In tendon segments incubated in chambers for three weeks, collagen synthesis was reduced by 50% and the rate of cell proliferation measured as 3H-thymidine incorporation, was 15 times that of native tendons. Frozen and incubated tendons showed only traces of remaining matrix synthesis or cell proliferation. With this experimental model we have histologically and biochemically shown that tendons may survive and heal while the nutrition exclusively could be based on diffusion and the tendons have an intrinsic capacity of healing. The described model enables further studies on tendon healing and its regulation.

KW - Cell proliferation

KW - Chamber

KW - Collagen

KW - Tendon healing

UR - http://www.scopus.com/inward/record.url?scp=0024784216&partnerID=8YFLogxK

U2 - 10.3109/02844318909075118

DO - 10.3109/02844318909075118

M3 - Article

C2 - 2617220

AN - SCOPUS:0024784216

VL - 23

SP - 199

EP - 205

JO - Journal of Plastic Surgery and Hand Surgery

JF - Journal of Plastic Surgery and Hand Surgery

SN - 2000-656X

IS - 3

ER -