The anandamide transport inhibitor AM404 activates vanilloid receptors

Research output: Contribution to journalArticle

Abstract

The possibility that the anandamide transport inhibitor N-(4-hydroxyphenyl)-5,8,11,14-eicosatetraenamide (AM404), structurally similar to the vanilloid receptor agonists anandamide and capsaicin, may also activate vanilloid receptors and cause vasodilation was examined. AM404 evoked concentration-dependent relaxations in segments of rat isolated hepatic artery contracted with phenylephrine. Relaxations were abolished in preparations pre-treated with capsaicin. The calcitonin-gene related peptide (CGRP) receptor antagonist CGRP-(8-37) also abolished relaxations. The vanilloid receptor antagonist capsazepine inhibited vasodilation by AM404 and blocked AM404-induced currents in patch-clamp experiments on Xenopus oocytes expressing the vanilloid subtype 1 receptor (VR1). In conclusion, AM404 activates native and cloned vanilloid receptors. Copyright (C) 2000 Elsevier Science B.V.

Details

Authors
Organisations
External organisations
  • University of California, San Francisco
  • Lund University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Pharmaceutical Sciences

Keywords

  • Afferent, Cannabinoid, Capsaicin, Neuron, Pain, Receptor, Sensory, Vasodilation
Original languageEnglish
Pages (from-to)39-42
Number of pages4
JournalEuropean Journal of Pharmacology
Volume396
Issue number1
Publication statusPublished - 2000 May 12
Publication categoryResearch
Peer-reviewedYes