The Application of the Drop Volume Technique to Measurements of the Adsorption of Proteins at Interfaces.

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T1 - The Application of the Drop Volume Technique to Measurements of the Adsorption of Proteins at Interfaces.

AU - Tornberg, Eva

PY - 1978

Y1 - 1978

N2 - A new procedure for the application of the drop volume technique to measurements of the rate of adsorption of proteins at interfaces has been developed. The mode of adsorption of the proteins lysozyme, β-lactoglobulin and bovine serum albumin (BSA) at the air-water interface has been measured with the drop volume method and has been compared to measurements with the Wilhelmy plate technique. Due to surface enlargement of the drop throughout the process of the surface tension decay, slower kinetics of the adsorption process is obtained by the drop volume method compared to the Wilhelmy plate technique, and the proteins investigated were differently sensitive to this surface expansion. The adsorption process of the proteins has been evaluated in terms of different rate-determining processes. Different intermediate states between the native and the denatured forms have been observed.

AB - A new procedure for the application of the drop volume technique to measurements of the rate of adsorption of proteins at interfaces has been developed. The mode of adsorption of the proteins lysozyme, β-lactoglobulin and bovine serum albumin (BSA) at the air-water interface has been measured with the drop volume method and has been compared to measurements with the Wilhelmy plate technique. Due to surface enlargement of the drop throughout the process of the surface tension decay, slower kinetics of the adsorption process is obtained by the drop volume method compared to the Wilhelmy plate technique, and the proteins investigated were differently sensitive to this surface expansion. The adsorption process of the proteins has been evaluated in terms of different rate-determining processes. Different intermediate states between the native and the denatured forms have been observed.

U2 - 10.1016/0021-9797(78)90382-X

DO - 10.1016/0021-9797(78)90382-X

M3 - Article

VL - 64

SP - 391

EP - 402

JO - Journal of Colloid and Interface Science

T2 - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

SN - 0021-9797

IS - 3

ER -