The CD53 and CEACAM-1 genes are genetic targets for early B cell factor.

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The CD53 and CEACAM-1 genes are genetic targets for early B cell factor. / Månsson, Robert; Lagergren, Anna; Hansson, Frida; Smith, Emma; Sigvardsson, Mikael.

In: European Journal of Immunology, Vol. 37, No. 5, 2007, p. 1365-1376.

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Månsson, Robert ; Lagergren, Anna ; Hansson, Frida ; Smith, Emma ; Sigvardsson, Mikael. / The CD53 and CEACAM-1 genes are genetic targets for early B cell factor. In: European Journal of Immunology. 2007 ; Vol. 37, No. 5. pp. 1365-1376.

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TY - JOUR

T1 - The CD53 and CEACAM-1 genes are genetic targets for early B cell factor.

AU - Månsson, Robert

AU - Lagergren, Anna

AU - Hansson, Frida

AU - Smith, Emma

AU - Sigvardsson, Mikael

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hematopoietic Stem Cell Laboratory (013022012)

PY - 2007

Y1 - 2007

N2 - Early B cell factor (EBF)-1 is a transcription factor known to be of critical importance for early B lymphocyte development. EBF-1 has been shown to directly interact with and regulate expression of a set of genes involved in the functional formation of the preB cell receptor, but the dramatic phenotype observed in the EBF-1-deficient mice suggests that several additional genes are activated by this protein. In order to identify additional target genes for EBF-1, we transduced a hematopoietic progenitor cell line, BaF/3, with an EBF-1-encoding retrovirus and investigated the induced gene expression pattern by micro-arrays. This analysis suggested that among others, the CD53 and the carcinoembryonic antigen-related cell adhesion molecule (CEACAM)-1 genes both were induced by ectopic expression of EBF-1. Identification of the 5' end of the cDNA enabled the identification of promoter elements with functional binding sites for EBF-1 and ability to respond to EBF-1 expression in transient transfection assays. These data suggest that CD53 and CEACAM-1 are direct genetic targets for EBF-1, providing additional information concerning the activity of this crucial transcription factor in hematopoiesis.

AB - Early B cell factor (EBF)-1 is a transcription factor known to be of critical importance for early B lymphocyte development. EBF-1 has been shown to directly interact with and regulate expression of a set of genes involved in the functional formation of the preB cell receptor, but the dramatic phenotype observed in the EBF-1-deficient mice suggests that several additional genes are activated by this protein. In order to identify additional target genes for EBF-1, we transduced a hematopoietic progenitor cell line, BaF/3, with an EBF-1-encoding retrovirus and investigated the induced gene expression pattern by micro-arrays. This analysis suggested that among others, the CD53 and the carcinoembryonic antigen-related cell adhesion molecule (CEACAM)-1 genes both were induced by ectopic expression of EBF-1. Identification of the 5' end of the cDNA enabled the identification of promoter elements with functional binding sites for EBF-1 and ability to respond to EBF-1 expression in transient transfection assays. These data suggest that CD53 and CEACAM-1 are direct genetic targets for EBF-1, providing additional information concerning the activity of this crucial transcription factor in hematopoiesis.

U2 - 10.1002/eji.200636642

DO - 10.1002/eji.200636642

M3 - Article

VL - 37

SP - 1365

EP - 1376

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 1521-4141

IS - 5

ER -