The copy number variation and stroke (CaNVAS) risk and outcome study

Research output: Contribution to journalArticle

Abstract

Background and purpose The role of copy number variation (CNV) variation in stroke susceptibility and outcome has yet to be explored. The Copy Number Variation and Stroke (CaNVAS) Risk and Outcome study addresses this knowledge gap. Methods Over 24,500 well-phenotyped IS cases, including IS subtypes, and over 43,500 controls have been identified, all with readily available genotyping on GWAS and exome arrays, with case measures of stroke outcome. To evaluate CNV-associated stroke risk and stroke outcome it is planned to: 1) perform Risk Discovery using several analytic approaches to identify CNVs that are associated with the risk of IS and its subtypes, across the age-, sex- and ethnicity-spectrums; 2) perform Risk Replication and Extension to determine whether the identified stroke-associated CNVs replicate in other ethnically diverse datasets and use biomarker data (e.g. methylation, proteomic, RNA, miRNA, etc.) to evaluate how the identified CNVs exert their effects on stroke risk, and lastly; 3) perform outcome-based Replication and Extension analyses of recent findings demonstrating an inverse relationship between CNV burden and stroke outcome at 3 months (mRS), and then determine the key CNV drivers responsible for these associations using existing biomarker data. Results The results of an initial CNV evaluation of 50 samples from each participating dataset are presented demonstrating that the existing GWAS and exome chip data are excellent for the planned CNV analyses. Further, some samples will require additional considerations for analysis, however such samples can readily be identified, as demonstrated by a sample demonstrating clonal mosaicism. Conclusion The CaNVAS study will cost-effectively leverage the numerous advantages of using existing case-control data sets, exploring the relationships between CNV and IS and its subtypes, and outcome at 3 months, in both men and women, in those of African and European-Caucasian descent, this, across the entire adult-age spectrum.

Details

Authors
  • John W Cole
  • Taiwo Adigun
  • Rufus Akinyemi
  • Onoja Matthew Akpa
  • Steven Bell
  • Bowang Chen
  • Jordi Jimenez Conde
  • Uxue Lazcano Dobao
  • Israel Fernandez
  • Myriam Fornage
  • Cristina Gallego-Fabrega
  • Christina Jern
  • Michael Krawczak
  • Hugh S. Markus
  • Mayowa Owolabi
  • Kristina Schlicht
  • Vinodh Srinivasasainagendra
  • Carolina Soriano Tárraga
  • Hemant Tiwari
  • Margaret Corasaniti
  • Natalie Fecteau
  • Beth Guizzardi
  • Haley Lopez
  • Kevin Nguyen
  • Brady Gaynor
  • Timothy O’Connor
  • O. Colin Stine
  • Steven J. Kittner
  • Patrick McArdle
  • Braxton D. Mitchell
  • Huichun Xu
  • Caspar Grond-Ginsbach
Organisations
External organisations
  • University of Maryland School of Medicine
  • University of Cambridge
  • Autonomous University of Barcelona
  • Hospital de Sant Pau
  • University of Texas
  • University of Gothenburg
  • University of Kiel
  • Skåne University Hospital
  • University of Alabama
  • Heidelberg University
  • University of Ibadan
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Neurology
Original languageEnglish
Article numbere0248791
JournalPLoS ONE
Volume16
Issue number4 April
Publication statusPublished - 2021
Publication categoryResearch
Peer-reviewedYes