The cultured neuroretina: development, survival and transplantation

Research output: ThesisDoctoral Thesis (compilation)

Abstract

In the cultured immature rat full-thickness neuroretina, massive apoptosis appears in the GCL within hours after explantation, but in the ONL cell death is low. Microglia cells become activated when the retina is kept in culture and are found in the GCL within 24 hours of culture.

Embryonic porcine retina kept in culture can survive and develop according to its intrinsic timetable for at least six weeks in vitro.

Short-term culture of embryonic donor tissue prior to transplantation does not seem to affect the differentiation and survival of the graft. A laminated graft can rescue rods in a large-animal model for severe retinitis pigmentosa.

Adult porcine retina kept in culture displays early degenerative changes, but many of the cells can survive for several days in the culture environment. Retinas kept in culture for up to 1 day can survive transplantation.

Human embryonic retinas can be kept in tissue culture for at least six weeks. They develop a partly laminated structure and several of the retina-specific neurons and glial cells.

Details

Authors
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Ophthalmology

Keywords

  • Oftalmologi, Ophtalmology, transplantation, development, culture, retina
Original languageEnglish
QualificationDoctor
Awarding Institution
Supervisors/Assistant supervisor
Award date2007 Mar 10
Publisher
  • Department of Ophthalmology, Lund University
Print ISBNs978-91-85559-19-8
Publication statusPublished - 2007
Publication categoryResearch

Bibliographic note

Defence details Date: 2007-03-10 Time: 10:00 Place: Segerfalksalen External reviewer(s) Name: Kvanta, Anders Title: MD, PhD Affiliation: S:t Eriks Ögonsjukhus ---