The establishment of 20 different human embryonic stem cell lines and subclones; a report on derivation, culture, characterisation and banking

Research output: Contribution to journalArticle

Abstract

This report summarises our efforts in deriving, characterising and banking of 20 different human embryonic stem cell lines. We have derived a large number of human embryonic stem cell lines between 2001 and 2005. One of these cell lines was established under totally xeno-free culture conditions. In addition, several subclones have been established, including a karyoptypical normal clone from a trisomic mother line. A master cell banking system has been utilised in concert with an extensive characterisation programme, ensuring a supply of high quality pluripotent stem cells for further research and development. In this report we also present the first data on a proprietary novel antibody, hES-Cellect, that exhibits high specificity for undifferentiated hES cells. In addition to the traditional manual dissection approach of propagating hES cells, we here also report on the successful approaches of feeder-free cultures as well as single cell cultures based on enzymatic digestion. All culture systems used as reported here have maintained the hES cells in a karyotypical normal and pluripotent state. These systems also have the advantage of being the principal springboards for further scale up of cultures for industrial or clinical applications that would require vastly more cells that can be produced by mechanical means.

Details

Authors
  • Mikael C. O. Englund
  • Gunilla Caisander
  • Karin Noaksson
  • Katarina Emanuelsson
  • Kersti Lundin
  • Christina Bergh
  • Charles Hansson
  • Henrik Semb
  • Raimund Strehl
  • Johan Hyllner
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology

Keywords

  • Cell banking, Characterisation, Pluripotency, Single cells, Feeder-free, Xeno-free, hES cells, Derivation
Original languageEnglish
Pages (from-to)217-230
JournalIn Vitro Cellular & Developmental Biology - Animal
Volume46
Issue number3-4
Publication statusPublished - 2010
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Stem Cell and Pancreas Developmental Biology (013212044)