The fat-like gene of drosophila is the true orthologue of vertebrate fat cadherins and is involved in the formation of tubular organs.

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The fat-like gene of drosophila is the true orthologue of vertebrate fat cadherins and is involved in the formation of tubular organs. / Castillejo-Lopez, Casimiro; Martinez Arias, Wilma; Baumgartner, Stefan.

In: Journal of Biological Chemistry, Vol. 279, No. 23, 2004, p. 24034-24043.

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T1 - The fat-like gene of drosophila is the true orthologue of vertebrate fat cadherins and is involved in the formation of tubular organs.

AU - Castillejo-Lopez, Casimiro

AU - Martinez Arias, Wilma

AU - Baumgartner, Stefan

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Developmental biology (LUR000007), Invertebral Developmental Biology, Udo Haecker's group (013212048), Invertebral Developmental Biology, Stefan Baumgartner's group (013212047)

PY - 2004

Y1 - 2004

N2 - Fat cadherins constitute a subclass of the large cadherin family characterized by the presence of 34 cadherin motifs. To date, three mammalian Fat cadherins have been described; however, only limited information is known about the function of these molecules. In this paper, we describe the second fat cadherin in Drosophila, fat-like (ftl). We show that ftl is the true orthologue of vertebrate fat-like genes, whereas the previously characterized tumor suppressor cadherin, fat, is more distantly related as compared with ftl. Ftl is a large molecule of 4705 amino acids. It is expressed apically in luminal tissues such as trachea, salivary glands, proventriculus, and hindgut. Silencing of ftl results in the collapse of tracheal epithelia giving rise to breaks, deletions, and sac-like structures. Other tubular organs such as proventriculus, salivary glands, and hindgut are also malformed or missing. These data suggest that Ftl is required for morphogenesis and maintenance of tubular structures of ectodermal origin and underline its similarity in function to a reported lethal mouse knock-out of fat1 where glomerular epithelial processes collapse. Based on our results, we propose a model where Ftl acts as a spacer to keep tubular epithelia apart rather than the previously described adhesive properties of the cadherin superfamily.

AB - Fat cadherins constitute a subclass of the large cadherin family characterized by the presence of 34 cadherin motifs. To date, three mammalian Fat cadherins have been described; however, only limited information is known about the function of these molecules. In this paper, we describe the second fat cadherin in Drosophila, fat-like (ftl). We show that ftl is the true orthologue of vertebrate fat-like genes, whereas the previously characterized tumor suppressor cadherin, fat, is more distantly related as compared with ftl. Ftl is a large molecule of 4705 amino acids. It is expressed apically in luminal tissues such as trachea, salivary glands, proventriculus, and hindgut. Silencing of ftl results in the collapse of tracheal epithelia giving rise to breaks, deletions, and sac-like structures. Other tubular organs such as proventriculus, salivary glands, and hindgut are also malformed or missing. These data suggest that Ftl is required for morphogenesis and maintenance of tubular structures of ectodermal origin and underline its similarity in function to a reported lethal mouse knock-out of fat1 where glomerular epithelial processes collapse. Based on our results, we propose a model where Ftl acts as a spacer to keep tubular epithelia apart rather than the previously described adhesive properties of the cadherin superfamily.

U2 - 10.1074/jbc.M313878200

DO - 10.1074/jbc.M313878200

M3 - Article

VL - 279

SP - 24034

EP - 24043

JO - Journal of Biological Chemistry

T2 - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 1083-351X

IS - 23

ER -