The inhibition of ammonium uptake in excised birch (Betula pendula) roots by batatasin-III.

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The inhibition of ammonium uptake in excised birch (Betula pendula) roots by batatasin-III. / Wallstedt, Anna; Sommarin, Marianne; Nilsson, Marie-Charlotte; Munson, Alison D.; Margolis, Hank A.

In: Physiologia Plantarum, Vol. 113, No. 3, 2001, p. 368-376.

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Wallstedt, A, Sommarin, M, Nilsson, M-C, Munson, AD & Margolis, HA 2001, 'The inhibition of ammonium uptake in excised birch (Betula pendula) roots by batatasin-III.', Physiologia Plantarum, vol. 113, no. 3, pp. 368-376. https://doi.org/10.1034/j.1399-3054.2001.1130310.x

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Wallstedt, Anna ; Sommarin, Marianne ; Nilsson, Marie-Charlotte ; Munson, Alison D. ; Margolis, Hank A. / The inhibition of ammonium uptake in excised birch (Betula pendula) roots by batatasin-III. In: Physiologia Plantarum. 2001 ; Vol. 113, No. 3. pp. 368-376.

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TY - JOUR

T1 - The inhibition of ammonium uptake in excised birch (Betula pendula) roots by batatasin-III.

AU - Wallstedt, Anna

AU - Sommarin, Marianne

AU - Nilsson, Marie-Charlotte

AU - Munson, Alison D.

AU - Margolis, Hank A.

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biochemistry and Structural Biology (S) (000006142), Chemical Ecology/Ecotoxicology (Closed 2011) (011006020)

PY - 2001

Y1 - 2001

N2 - In northern Sweden, plants growing in association with the clonal dwarf shrub Empetrum hermaphroditum usually exhibit limited growth and are N-depleted. Previous studies suggest that this negative effect by E. hermaphroditum may be explained, at least in part, by the release of phenolic compounds, particularly the dihydrostilbene, batatasin-III from foliage to soil. In the present work, we investigated whether batatasin-III has the potential to interfere with NH4+ uptake in birch (Betula pendula) roots. Excised birch roots were exposed to batatasin-III during brief periods in 15NH4+ solutions, and then analyzed for labeled N. Batatasin-III inhibited N-NH4+ uptake by 28, 89 and 95% compared with the control, when roots were treated with 0.1, 1.0 and 2.8 mM of batatasin-III, respectively. The effect of 1.0-mM batatasin-III was greater at pH 4.2 than at pH 6.8. In addition, the inhibition of N-NH4+ uptake by batatasin-III was not reversed after rinsing the roots in water and transferring them to a batatasin-III free solution. Furthermore, birch seedlings immersed in a 1.0-mM batatasin-III solution for 2 h, and then replanted in pots with soil, had decreased growth, such that 10 weeks after treatment, the dry mass of both shoots and roots was reduced by 74 and 73%, respectively, compared with control seedlings. This suggests that a brief exposure to batatasin-III may have a long-term inhibitory effect on whole plant growth. Using plasma membrane vesicles isolated from easily extractable spinach (Spinacia oleracea) leaves, it was found that batatasin-III strongly inhibited proton pumping in isolated plasma membrane vesicles, while it only slightly inhibited ATP hydrolytic activity. The uncoupling of proton pumping from ATP hydrolytic activity suggests that batatasin-III disturbs membrane integrity. This hypothesis was further supported by a greater efflux of ions from birch roots immersed in a batatasin-III solution than from roots in a control solution.

AB - In northern Sweden, plants growing in association with the clonal dwarf shrub Empetrum hermaphroditum usually exhibit limited growth and are N-depleted. Previous studies suggest that this negative effect by E. hermaphroditum may be explained, at least in part, by the release of phenolic compounds, particularly the dihydrostilbene, batatasin-III from foliage to soil. In the present work, we investigated whether batatasin-III has the potential to interfere with NH4+ uptake in birch (Betula pendula) roots. Excised birch roots were exposed to batatasin-III during brief periods in 15NH4+ solutions, and then analyzed for labeled N. Batatasin-III inhibited N-NH4+ uptake by 28, 89 and 95% compared with the control, when roots were treated with 0.1, 1.0 and 2.8 mM of batatasin-III, respectively. The effect of 1.0-mM batatasin-III was greater at pH 4.2 than at pH 6.8. In addition, the inhibition of N-NH4+ uptake by batatasin-III was not reversed after rinsing the roots in water and transferring them to a batatasin-III free solution. Furthermore, birch seedlings immersed in a 1.0-mM batatasin-III solution for 2 h, and then replanted in pots with soil, had decreased growth, such that 10 weeks after treatment, the dry mass of both shoots and roots was reduced by 74 and 73%, respectively, compared with control seedlings. This suggests that a brief exposure to batatasin-III may have a long-term inhibitory effect on whole plant growth. Using plasma membrane vesicles isolated from easily extractable spinach (Spinacia oleracea) leaves, it was found that batatasin-III strongly inhibited proton pumping in isolated plasma membrane vesicles, while it only slightly inhibited ATP hydrolytic activity. The uncoupling of proton pumping from ATP hydrolytic activity suggests that batatasin-III disturbs membrane integrity. This hypothesis was further supported by a greater efflux of ions from birch roots immersed in a batatasin-III solution than from roots in a control solution.

U2 - 10.1034/j.1399-3054.2001.1130310.x

DO - 10.1034/j.1399-3054.2001.1130310.x

M3 - Article

VL - 113

SP - 368

EP - 376

JO - Physiologia Plantarum

JF - Physiologia Plantarum

SN - 0031-9317

IS - 3

ER -