The interactive effect of an AM fungus and an organic amendment with regard to improving inoculum potential and the growth and nutrition of Trifolium repens in Cd-contaminated soils

Research output: Contribution to journalArticle


Aspergillus niger-treated dry olive cake (DryOC) can be used as a soil organic amendment and the aim of this work was to study the effectiveness of this amendment and a Cd-adapted arbuscular mycorrhizal (AM) fungus in improving Trifolium repens growth and nutrition in Cd-contaminated soil. In a compartmentalized growth system, consisting of a root compartment (RC) and two hyphal compartments (HCs), we investigated the influence of the amendment on intraradical and extraradical AM fungi development. In addition, we studied the viability and infectivity of the detached extraradical mycelium in plants, designated as receptor plants, grown in the HC after removal of the RC. Both the amendment and the AM fungus increased shoot and root biomass and nodulation in both the non-contaminated and Cd-contaminated soils. The positive interaction between the microbiologically treated DryOC and the AM fungus resulted in the highest plant yield, which can be explained by enhanced nutrient acquisition and arbuscular richness as well as by the immobilisation of Cd in amended soils. However, A. niger-treated DryOC had no effect on the extraradical mycorrhizal mycelium development. Although Cd decreased AM hyphal length density, symbiotic infectivity was similar in receptor plants grown in non-contaminated and contaminated soil, thus confirming the AM fungal inoculum potential. The combination of the AM fungus and A. niger-treated DryOC increased plant tolerance to Cd in terms of plant growth and nutrition and can be regarded as an important strategy for reclaiming Cd-contaminated soils. (C) 2009 Elsevier B.V. All rights reserved.


  • Almudena Medina
  • N. Vassilev
  • R. Azcon
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Ecology


  • contamination, Cd, Organic amendment, Glomus mosseae, Arbuscular mycorrhizal fungi, AM extraradical mycelium growth
Original languageEnglish
Pages (from-to)181-189
JournalApplied Soil Ecology
Issue number2
Publication statusPublished - 2010
Publication categoryResearch

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Plant Ecology and Systematics (Closed 2011) (011004000)