The Lecticans of Mammalian Brain Perineural Net Are O-Mannosylated

Research output: Contribution to journalArticle


O-Mannosylation is an important protein modification in brain. During the last years, a few mammalian proteins have been identified as targets of the protein-O-mannosyltransferases 1 and 2. However, these still cannot explain the high content of O-mannosyl glycans in brain and the strong brain involvement of congenital muscular dystrophies caused by POMT mutations (Walker-Warburg syndrome, dystroglycanopathies). By fractionating and analyzing the glycoproteome of mouse and calf brain lysates, we could show that proteins of the perineural net, the lecticans, are O-mannosylated, indicating that major components of neuronal extracellular matrix are O-mannosylated in mammalian brain. This finding corresponds with the high content of O-mannosyl glycans in brain as well as with the brain involvement of dystroglycanopathies. In contrast, the lectican neurocan is not O-mannosylated when recombinantly expressed in EBNA-293 cells, revealing the possibility of different control mechanisms for the initiation of O-mannosylation in different cell types.


  • Sandra Pacharra
  • Franz-Georg Hanisch
  • Martina Muehlenhoff
  • Andreas Faissner
  • Uwe Rauch
  • Isabelle Breloy
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology


  • O-glycans, O-mannosylation, lecticans, ECM, perineural net, O-glycosylation, ESI-MS/MS, MALDI-MS/MS, glycoproteomics, dystroglycanopathies
Original languageEnglish
Pages (from-to)1764-1771
JournalJournal of Proteome Research
Issue number4
Publication statusPublished - 2013
Publication categoryResearch