The Lecticans of Mammalian Brain Perineural Net Are O-Mannosylated

Research output: Contribution to journalArticle


title = "The Lecticans of Mammalian Brain Perineural Net Are O-Mannosylated",
abstract = "O-Mannosylation is an important protein modification in brain. During the last years, a few mammalian proteins have been identified as targets of the protein-O-mannosyltransferases 1 and 2. However, these still cannot explain the high content of O-mannosyl glycans in brain and the strong brain involvement of congenital muscular dystrophies caused by POMT mutations (Walker-Warburg syndrome, dystroglycanopathies). By fractionating and analyzing the glycoproteome of mouse and calf brain lysates, we could show that proteins of the perineural net, the lecticans, are O-mannosylated, indicating that major components of neuronal extracellular matrix are O-mannosylated in mammalian brain. This finding corresponds with the high content of O-mannosyl glycans in brain as well as with the brain involvement of dystroglycanopathies. In contrast, the lectican neurocan is not O-mannosylated when recombinantly expressed in EBNA-293 cells, revealing the possibility of different control mechanisms for the initiation of O-mannosylation in different cell types.",
keywords = "O-glycans, O-mannosylation, lecticans, ECM, perineural net, O-glycosylation, ESI-MS/MS, MALDI-MS/MS, glycoproteomics, dystroglycanopathies",
author = "Sandra Pacharra and Franz-Georg Hanisch and Martina Muehlenhoff and Andreas Faissner and Uwe Rauch and Isabelle Breloy",
year = "2013",
doi = "10.1021/pr3011028",
language = "English",
volume = "12",
pages = "1764--1771",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "The American Chemical Society (ACS)",
number = "4",