The N-terminal Ankyrin Repeat domain is not required for electrophile and heat activation of the purified mosquito TRPA1 receptor

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Abstract

Temperature sensors are crucial for animals to optimize living conditions. The temperature response of the ion channel transient receptor potential A1 (TRPA1) is intriguing; some orthologs have been reported to be activated by cold and others by heat, but the molecular mechanisms responsible for its activation remain elusive. Single-channel electrophysiological recordings of heterologously expressed and purified Anopheles gambiae TRPA1 (AgTRPA1), with and without the N-terminal ankyrin repeat domain, demonstrate that both proteins are functional because they responded to the electrophilic compounds allyl isothiocyanate and cinnamaldehyde as well as heat. The proteins' similar intrinsic fluorescence properties and corresponding quenching when activated by allyl isothiocyanate or heat suggest lipid bilayer-independent conformational changes outside the N-terminal domain. The results show that Ag- TRPA1 is an inherent thermo- and chemoreceptor, and analogous to what has been reported for the human TRPA1 ortholog, the N-terminal domain may tune the response but is not required for the activation by these stimuli.

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  • Biochemistry and Molecular Biology
Original languageEnglish
Pages (from-to)26899-26912
Number of pages14
JournalJournal of Biological Chemistry
Volume291
Issue number52
Publication statusPublished - 2016 Dec 23
Publication categoryResearch
Peer-reviewedYes