The PEF family proteins sorcin and grancalcin interact in vivo and in vitro

Research output: Contribution to journalArticle

Abstract

The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.

Details

Authors
  • Christian Hansen
  • Svetlana Tarabykina
  • Jonas M. La Cour
  • Karsten Lollike
  • Martin W. Berchtold
External organisations
  • University of Copenhagen
Research areas and keywords

Keywords

  • Apoptosis, EF hand calcium binding protein, Grancalcin, Penta-EF hand family protein, Protein-protein interaction, Sorcin
Original languageEnglish
Pages (from-to)151-154
Number of pages4
JournalFEBS Letters
Volume545
Issue number2-3
Publication statusPublished - 2003 Jun 19
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes