The quantitative proteome of a human cell line

Research output: Contribution to journalArticle

Abstract

The generation of mathematical models of biological processes, the simulation of these processes under different conditions, and the comparison and integration of multiple data sets are explicit goals of systems biology that require the knowledge of the absolute quantity of the system's components. To date, systematic estimates of cellular protein concentrations have been exceptionally scarce. Here, we provide a quantitative description of the proteome of a commonly used human cell line in two functional states, interphase and mitosis. We show that these human cultured cells express at least similar to 10 000 proteins and that the quantified proteins span a concentration range of seven orders of magnitude up to 20 000 000 copies per cell. We discuss how protein abundance is linked to function and evolution. Molecular Systems Biology 7: 549; published online 8 November 2011; doi:10.1038/msb.2011.82

Details

Authors
  • Martin Beck
  • Alexander Schmidt
  • Johan Malmström
  • Manfred Claassen
  • Alessandro Ori
  • Anna Szymborska
  • Franz Herzog
  • Oliver Rinner
  • Jan Ellenberg
  • Ruedi Aebersold
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area

Keywords

  • mass spectrometry, protein abundance, proteomics
Original languageEnglish
JournalMolecular Systems Biology
Volume7
Publication statusPublished - 2011
Publication categoryResearch
Peer-reviewedYes