The serine protease Sp7 is expressed in blood cells and regulates the melanization reaction in Drosophila.

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The serine protease Sp7 is expressed in blood cells and regulates the melanization reaction in Drosophila. / Castillejo-Lopez, Casimiro; Häcker, Udo.

In: Biochemical and Biophysical Research Communications, Vol. 338, No. 2, 2005, p. 1075-1082.

Research output: Contribution to journalArticle

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TY - JOUR

T1 - The serine protease Sp7 is expressed in blood cells and regulates the melanization reaction in Drosophila.

AU - Castillejo-Lopez, Casimiro

AU - Häcker, Udo

PY - 2005

Y1 - 2005

N2 - Serine proteases play a central role in defense against pathogens by regulating processes such as blood clotting, melanization of injured surfaces, and proteolytic activation of signaling pathways involved in innate immunity. Here, we present the functional characterization of the Drosophila serine protease Sp7 (CG3006) by inducible RNA interference. We show that Sp7 is constitutively expressed in blood cells during embryonic and larval stages. Silencing of the gene impairs the melanization reaction upon injury. Our data demonstrate that Sp7 is required for phenoloxidase activation and its activity is restricted to a subclass of blood cells, the crystal cells. Transcriptional up-regulation of Sp7 was observed after clean, septic injury and in flies expressing an activated form of Toll; however, mutations in the Toll or the IMD pathway did not abolish expression of Sp7, indicating the existence of other regulatory pathways and/or independent basal transcription.

AB - Serine proteases play a central role in defense against pathogens by regulating processes such as blood clotting, melanization of injured surfaces, and proteolytic activation of signaling pathways involved in innate immunity. Here, we present the functional characterization of the Drosophila serine protease Sp7 (CG3006) by inducible RNA interference. We show that Sp7 is constitutively expressed in blood cells during embryonic and larval stages. Silencing of the gene impairs the melanization reaction upon injury. Our data demonstrate that Sp7 is required for phenoloxidase activation and its activity is restricted to a subclass of blood cells, the crystal cells. Transcriptional up-regulation of Sp7 was observed after clean, septic injury and in flies expressing an activated form of Toll; however, mutations in the Toll or the IMD pathway did not abolish expression of Sp7, indicating the existence of other regulatory pathways and/or independent basal transcription.

KW - Drosophila

KW - RNAi

KW - Serine protease

KW - Melanization

KW - Phenoloxidase

KW - Wound

KW - Blood cells

KW - Innate immunity

U2 - 10.1016/j.bbrc.2005.10.042

DO - 10.1016/j.bbrc.2005.10.042

M3 - Article

VL - 338

SP - 1075

EP - 1082

JO - Biochemical and Biophysical Research Communications

T2 - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 1090-2104

IS - 2

ER -