The significance of batch and patch test method in establishing contact allergy to fragrance mix I—EDEN Fragrance Study Group

Research output: Contribution to journalArticle


Background: A fragrance mix consisting of eight separate fragrance ingredients (fragrance mix I [FM I]) is present in most baseline patch test series. Patch testing with the TRUE Test technique is considered to detect less contact allergy to FM I than testing with the Finn Chamber technique. Objective: To investigate the possible significance of batch and patch test method in establishing contact allergy to FM I. Methods: Three thousand one hundred and nineteen individuals representing a sample of the general population were patch tested with two batches of FM I with two patch test techniques at six dermatology clinics in five European countries. The TRUE Test technique and the Finn Chamber technique with pet. preparations were used. McNemar's test was used for statistical calculations. Results: The contact allergy prevalences varied between 0.7% and 2.6%. The patch tests with the mixes containing Evernia prunastri (oak moss) with a high content of chloratranol/atranol resulted in substantially more positive reactions than the corresponding tests with the mixes containing oak moss with a low content of chloratranol/atranol. The Finn Chamber technique detected significantly more contact allergic reactions than the TRUE Test technique (P < 0.001). Conclusion: The Finn Chamber technique detects more contact allergy to FM I than the TRUE Test technique.


External organisations
  • Skåne University Hospital
  • Heidelberg University
  • University Medical Center Groningen
  • Universitätsklinikum Jena
  • University of Coimbra
  • Azienda Ospedaliera Papa Giovanni XXIII
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Dermatology and Venereal Diseases


  • allergic contact dermatitis, chloratranol/atranol, delayed hypersensitivity, Evernia prunastri, general population, oak moss
Original languageEnglish
Pages (from-to)104-109
Number of pages6
JournalContact Dermatitis
Issue number1
Early online date2019
Publication statusPublished - 2019 Aug
Publication categoryResearch