The Transcriptional Activity of Neurog3 Affects Migration and Differentiation of Ectopic Endocrine Cells in Chicken Endoderm

Research output: Contribution to journalArticle

Abstract

Neurog3 is expressed transiently in pancreatic endocrine progenitors where it is responsible for activating a transcription factor cascade which eventually defines the mature endocrine cells. However, the mechanism by which Neurog3 regulates different aspects of the endocrine differentiation program is less clear. In this report we used in ovo electroporation to investigate how manipulation of Neurog3 protein activity affected migration, differentiation and fate determination. We found that changes in the onset of Neurog3 expression only had minor effect on differentiation. However increasing the transcriptional activity of Neurog3 by fusing it to VP16 or co-electroporating with Ep300 caused the electroporated cells to migrate rather than differentiate. In contrast, reducing the transcriptional activity of Neurog3 by deleting parts of the activation domain, by fusing Neurog3 to the engrailed repressor domain, or co-electroporating with Hdac1 greatly increased the proportion of glucagon expressing cells. Developmental Dynamics 239:1950-1966, 2010. (C) 2010 Wiley-Liss, Inc.

Details

Authors
  • Louise C. Rosenberg
  • Merete L. Lafon
  • Jesper Karup
  • Hani Yassin
  • Jan Nygaard Jensen
  • Palle Serup
  • Jacob Hecksher-Sorensen
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology

Keywords

  • endocrine competence, glucagon, differentiation, pancreas, fluorescence, whole-mount, in ovo electroporation, neurogenin 3, Neurog3, Ngn3
Original languageEnglish
Pages (from-to)1950-1966
JournalDevelopmental Dynamics
Volume239
Issue number7
Publication statusPublished - 2010
Publication categoryResearch
Peer-reviewedYes