Thousand-Fold Volumetric Concentration of Live Cells with a Recirculating Acoustofluidic Device.

Research output: Contribution to journalArticle

Abstract

The ability to concentrate cells from dilute samples into smaller volumes is an essential process step for most biological assays. Volumetric concentration is typically achieved via centrifugation, but this technique is not well suited for handling small number of cells, especially outside of the laboratory setting. In this work, we describe a novel device that combines acoustofluidics with a recirculating architecture to achieve >1000-fold enrichment of cells in a label-free manner, at high volumetric throughput (>500 μL min(-1)) and with high recovery (>98.7%). We demonstrate that our device can be used with a wide variety of different cell types and show that this concentration strategy does not affect cell viability. Importantly, our device could be readily adopted to serve as a "sample preparation" module that can be integrated with other microfluidic devices to allow analysis of dilute cellular samples in large volumes.

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Subject classification (UKÄ) – MANDATORY

  • Analytical Chemistry
Original languageEnglish
Pages (from-to)8497-8502
JournalAnalytical Chemistry
Volume87
Issue number16
Publication statusPublished - 2015
Publication categoryResearch
Peer-reviewedYes

Related research output

Jakobsson, O., 2016 Oct 17, Lund: Department of Biomedical Engineering, Lund university. 123 p.

Research output: ThesisDoctoral Thesis (compilation)

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