Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration

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Bibtex

@article{d55b290655d54e0487b3bc296d6e41e7,
title = "Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration",
abstract = "Acellular scaffolds from complex whole organs such as lung are being increasingly studied for ex vivo organ generation and for in vitro studies of cell-extracellular matrix interactions. We have established effective methods for efficient de and recellularization of large animal and human lungs including techniques which allow multiple small segments (~1-3 cm3) to be excised that retain 3-dimensional lung structure. Coupled with the use of a synthetic pleural coating, cells can be selectively physiologically inoculated via preserved vascular and airway conduits. Inoculated segments can be further sliced for high throughput studies. Further, we demonstrate thermography as a powerful noninvasive technique for monitoring perfusion decellularization and for evaluating preservation of vascular and airway networks following human and porcine lung decellularization. Collectively, these techniques are a significant step forward as they allow high throughput in vitro studies from a single lung or lobe in a more biologically relevant, three-dimensional acellular scaffold.",
keywords = "Acellular matrix, Endothelial cell, Epithelial cell, Extracellular matrix (ECM), Human lung fibroblast, Mesenchymal stem cell",
author = "Wagner, {Darcy E.} and Bonenfant, {Nicholas R.} and Dino Sokocevic and DeSarno, {Michael J} and Borg, {Zachary D.} and Parsons, {Charles S.} and Brooks, {Elice M.} and Joseph Platz and Khalpey, {Zain I.} and Hoganson, {David M.} and Bin Deng and Lam, {Ying Wai} and Oldinski, {Rachael A.} and Takamaru Ashikaga and Weiss, {Daniel J.}",
year = "2014",
month = "3",
doi = "10.1016/j.biomaterials.2013.11.078",
language = "English",
volume = "35",
pages = "2664--2679",
journal = "Biomaterials",
issn = "1878-5905",
publisher = "Elsevier",
number = "9",

}