Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration

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Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration. / Wagner, Darcy E.; Bonenfant, Nicholas R.; Sokocevic, Dino; DeSarno, Michael J; Borg, Zachary D.; Parsons, Charles S.; Brooks, Elice M.; Platz, Joseph; Khalpey, Zain I.; Hoganson, David M.; Deng, Bin; Lam, Ying Wai; Oldinski, Rachael A.; Ashikaga, Takamaru; Weiss, Daniel J.

In: Biomaterials, Vol. 35, No. 9, 03.2014, p. 2664-2679.

Research output: Contribution to journalArticle

Harvard

Wagner, DE, Bonenfant, NR, Sokocevic, D, DeSarno, MJ, Borg, ZD, Parsons, CS, Brooks, EM, Platz, J, Khalpey, ZI, Hoganson, DM, Deng, B, Lam, YW, Oldinski, RA, Ashikaga, T & Weiss, DJ 2014, 'Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration', Biomaterials, vol. 35, no. 9, pp. 2664-2679. https://doi.org/10.1016/j.biomaterials.2013.11.078

APA

CBE

Wagner DE, Bonenfant NR, Sokocevic D, DeSarno MJ, Borg ZD, Parsons CS, Brooks EM, Platz J, Khalpey ZI, Hoganson DM, Deng B, Lam YW, Oldinski RA, Ashikaga T, Weiss DJ. 2014. Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration. Biomaterials. 35(9):2664-2679. https://doi.org/10.1016/j.biomaterials.2013.11.078

MLA

Vancouver

Author

Wagner, Darcy E. ; Bonenfant, Nicholas R. ; Sokocevic, Dino ; DeSarno, Michael J ; Borg, Zachary D. ; Parsons, Charles S. ; Brooks, Elice M. ; Platz, Joseph ; Khalpey, Zain I. ; Hoganson, David M. ; Deng, Bin ; Lam, Ying Wai ; Oldinski, Rachael A. ; Ashikaga, Takamaru ; Weiss, Daniel J. / Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration. In: Biomaterials. 2014 ; Vol. 35, No. 9. pp. 2664-2679.

RIS

TY - JOUR

T1 - Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration

AU - Wagner, Darcy E.

AU - Bonenfant, Nicholas R.

AU - Sokocevic, Dino

AU - DeSarno, Michael J

AU - Borg, Zachary D.

AU - Parsons, Charles S.

AU - Brooks, Elice M.

AU - Platz, Joseph

AU - Khalpey, Zain I.

AU - Hoganson, David M.

AU - Deng, Bin

AU - Lam, Ying Wai

AU - Oldinski, Rachael A.

AU - Ashikaga, Takamaru

AU - Weiss, Daniel J.

PY - 2014/3

Y1 - 2014/3

N2 - Acellular scaffolds from complex whole organs such as lung are being increasingly studied for ex vivo organ generation and for in vitro studies of cell-extracellular matrix interactions. We have established effective methods for efficient de and recellularization of large animal and human lungs including techniques which allow multiple small segments (~1-3 cm3) to be excised that retain 3-dimensional lung structure. Coupled with the use of a synthetic pleural coating, cells can be selectively physiologically inoculated via preserved vascular and airway conduits. Inoculated segments can be further sliced for high throughput studies. Further, we demonstrate thermography as a powerful noninvasive technique for monitoring perfusion decellularization and for evaluating preservation of vascular and airway networks following human and porcine lung decellularization. Collectively, these techniques are a significant step forward as they allow high throughput in vitro studies from a single lung or lobe in a more biologically relevant, three-dimensional acellular scaffold.

AB - Acellular scaffolds from complex whole organs such as lung are being increasingly studied for ex vivo organ generation and for in vitro studies of cell-extracellular matrix interactions. We have established effective methods for efficient de and recellularization of large animal and human lungs including techniques which allow multiple small segments (~1-3 cm3) to be excised that retain 3-dimensional lung structure. Coupled with the use of a synthetic pleural coating, cells can be selectively physiologically inoculated via preserved vascular and airway conduits. Inoculated segments can be further sliced for high throughput studies. Further, we demonstrate thermography as a powerful noninvasive technique for monitoring perfusion decellularization and for evaluating preservation of vascular and airway networks following human and porcine lung decellularization. Collectively, these techniques are a significant step forward as they allow high throughput in vitro studies from a single lung or lobe in a more biologically relevant, three-dimensional acellular scaffold.

KW - Acellular matrix

KW - Endothelial cell

KW - Epithelial cell

KW - Extracellular matrix (ECM)

KW - Human lung fibroblast

KW - Mesenchymal stem cell

UR - http://www.scopus.com/inward/record.url?scp=84895027459&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2013.11.078

DO - 10.1016/j.biomaterials.2013.11.078

M3 - Article

VL - 35

SP - 2664

EP - 2679

JO - Biomaterials

T2 - Biomaterials

JF - Biomaterials

SN - 1878-5905

IS - 9

ER -