Timing the doxycycline yields different patterns of genomic recombination in brain neurons with a new inducible Cre transgene.

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Timing the doxycycline yields different patterns of genomic recombination in brain neurons with a new inducible Cre transgene. / Lindeberg, J; Mattsson, Ragnar; Ebendal, T.

In: Journal of Neuroscience Research, Vol. 68, No. 2, 2002, p. 248-253.

Research output: Contribution to journalArticle

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TY - JOUR

T1 - Timing the doxycycline yields different patterns of genomic recombination in brain neurons with a new inducible Cre transgene.

AU - Lindeberg, J

AU - Mattsson, Ragnar

AU - Ebendal, T

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Medical Inflammation Research (013212019)

PY - 2002

Y1 - 2002

N2 - We have developed a transgenic mouse expressing the Cre recombinase under control of a tetracycline-responsive promoter. Using a CamKIIalpha-driven tTA transgenic strain and a lacZ reporter mouse, we obtained the expected neuronal pattern of recombination in the olfactory lobe, cortex, striatum, hippocampus and Purkinje cells. Moreover, recombination can be completely abolished by feeding the mice doxycycline in their drinking water. We also show that it is possible to get a different pattern of recombination by changing the timing of the doxycycline-mediated shutdown of Cre expression. By starting the doxycycline treatment at birth, we restrict recombination to striatum only. This approach should be applicable to other inducible transgenic strains, thus increasing the number of available tissue-specific patterns for conditional knockouts. Also, our tetO-Cre transgene can be combined with any of the increasing number of tetracycline transactivator transgenic strains to direct specifically inducible genomic recombination to several areas of the brain.

AB - We have developed a transgenic mouse expressing the Cre recombinase under control of a tetracycline-responsive promoter. Using a CamKIIalpha-driven tTA transgenic strain and a lacZ reporter mouse, we obtained the expected neuronal pattern of recombination in the olfactory lobe, cortex, striatum, hippocampus and Purkinje cells. Moreover, recombination can be completely abolished by feeding the mice doxycycline in their drinking water. We also show that it is possible to get a different pattern of recombination by changing the timing of the doxycycline-mediated shutdown of Cre expression. By starting the doxycycline treatment at birth, we restrict recombination to striatum only. This approach should be applicable to other inducible transgenic strains, thus increasing the number of available tissue-specific patterns for conditional knockouts. Also, our tetO-Cre transgene can be combined with any of the increasing number of tetracycline transactivator transgenic strains to direct specifically inducible genomic recombination to several areas of the brain.

U2 - 10.1002/jnr.10213

DO - 10.1002/jnr.10213

M3 - Article

VL - 68

SP - 248

EP - 253

JO - Journal of Neuroscience Research

T2 - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 1097-4547

IS - 2

ER -