Transgenic rescue demonstrates involvement of the Ian5 gene in T cell development in the rat.

Research output: Contribution to journalArticle

Abstract

A single point mutation in a novel immune-associated nucleotide gene 5 (Ian5) coincides with severe T cell lymphopenia in BB rats. We used a transgenic rescue approach in lymphopenic BB-derived congenic F344.lyp/lyp rats to determine whether this mutation is responsible for lymphopenia and to establish the functional importance of this novel gene. A 150-kb P1 artificial chromosome (PAC) transgene harboring a wild-type allele of the rat Ian5 gene restored Ian5 transcript and protein levels, completely rescuing the T cell lymphopenia in the F344.lyp/lyp rats. This successful complementation provides direct functional evidence that the Ian5 gene product is essential for maintaining normal T cell levels. It also demonstrates that transgenic rescue in the rat is a practical and definitive method for revealing the function of a novel gene.

Details

Authors
  • Mieczyslaw Michalkiewicz
  • Teresa Michalkiewicz
  • Ruth A. Ettinger
  • Elizabeth A. Rutledge
  • Jessica M. Fuller
  • Daniel H. Moralejo
  • Brian Van Yserloo
  • Armand J. MacMurray
  • Anne E. Kwitek
  • Howard J. Jacob
  • Eric S. Lander
  • Åke Lernmark
External organisations
  • University of Washington, Seattle
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Endocrinology and Diabetes
Original languageEnglish
Pages (from-to)228-232
JournalPhysiological Genomics
Volume19
Issue number2
Publication statusPublished - 2004
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes