Transgenic systems for unequivocal identification of cardiac myocyte nuclei and analysis of cardiomyocyte cell cycle status

Research output: Contribution to journalArticle

Abstract

Even though the mammalian heart has been investigated for many years, there are still uncertainties in the fields of cardiac cell biology and regeneration with regard to exact fractions of cardiomyocytes (CMs) at different developmental stages, their plasticity after cardiac lesion and also their basal turnover rate. A main shortcoming is the accurate identification of CM and the demonstration of CM division. Therefore, an in vivo model taking advantage of a live reporter-based identification of CM nuclei and their cell cycle status is needed. In this technical report, we describe the generation and characterization of embryonic stem cells and transgenic mice expressing a fusion protein of human histone 2B and the red fluorescence protein mCherry under control of the CM specific alpha MHC promoter. This fluorescence label allows unequivocal identification and quantitation of CM nuclei and nuclearity in isolated cells and native tissue slices. In ventricles of adults, we determined a fraction of <20 % CMs and binucleation of 77-90 %, while in atria a CM fraction of 30 % and a binucleation index of 14 % were found. We combined this transgenic system with the CAG-eGFP-anillin transgene, which identifies cell division and established a novel screening assay for cell cycle-modifying substances in isolated, postnatal CMs. Our transgenic live reporter-based system enables reliable identification of CM nuclei and determination of CM fractions and nuclearity in heart tissue. In combination with CAG-eGFP-anillin-mice, the cell cycle status of CMs can be monitored in detail enabling screening for proliferation-inducing substances in vitro and in vivo.

Details

Authors
  • Alexandra Raulf
  • Hannes Horder
  • Laura Tarnawski
  • Caroline Geisen
  • Annika Ottersbach
  • Wilhelm Roell
  • Stefan Jovinge
  • Bernd K. Fleischmann
  • Michael Hesse
Organisations
External organisations
  • Van Andel Research Institute
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cardiac and Cardiovascular Systems

Keywords

  • Genetically altered mice, Cardiac myocyte, Cell cycle, Proliferation, miRNA
Original languageEnglish
Pages (from-to)33
JournalBasic Research in Cardiology
Volume110
Issue number3
Publication statusPublished - 2015
Publication categoryResearch
Peer-reviewedYes

Related research output

Tarnawski, L., 2015, Stem Cell Center, Lund University. 45 p.

Research output: ThesisDoctoral Thesis (compilation)

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