Transient Aggregates in Protein Folding are Easily Mistaken for Folding Intermediates

Research output: Contribution to journalArticle

Abstract

It has been questioned recently whether populated intermediates are important for the protein folding process or are artefacts trapped in nonproductive pathways. We report here that the rapidly formed intermediate of the spliceosomal protein U1A is an off-pathway artefact caused by transient aggregation of denatured protein under native conditions. Transient aggregates are easily mistaken for structured monomers and could be a general problem in time-resolved folding studies.

Details

Authors
  • Maria Silow
  • Mikael Oliveberg
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biological Sciences

Keywords

  • Biochemistry, U1A spliceosomal protein, Aggregation artefacts, Off-pathway intermediates
Original languageEnglish
Pages (from-to)6084-6086
JournalProceedings of the National Academy of Sciences
Volume94
Issue number12
Publication statusPublished - 1997
Publication categoryResearch
Peer-reviewedYes