Transient inhibition of NF-κB signaling enhances ex vivo propagation of human hematopoietic stem cells

Research output: Contribution to journalArticle

Abstract

Despite extensive studies, defining culture conditions in which hematopoietic stem cells can be expanded ex vivo has been challenging. Here we show that chemical inhibition of the NF- κB signaling pathway leads to a significant improvement of hematopoietic stem cell function from ex vivo cultured human umbilical cord blood derived CD34+ cells. We found a distinct peak of activation of the NF-κB pathway shortly after cells were put in culture, and consequently inhibition of the pathway was both necessary and sufficient during the first 24 hours of culture where it reduced the levels of several pro-inflammatory cytokines. Taken together, NF-κB pathway inhibition facilitates propagation of hematopoietic stem cells in culture and may complement other strategies for hematopoietic stem cell expansion by relieving stress signals that are induced as an immediate response to culture initiation.

Details

Authors
Organisations
External organisations
  • Lund University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Hematology
  • Cell and Molecular Biology
Original languageEnglish
Pages (from-to)1444-1450
Number of pages7
JournalHaematologica
Volume103
Issue number9
Publication statusPublished - 2018
Publication categoryResearch
Peer-reviewedYes