TRIM28 repression of retrotransposon-based enhancers is necessary to preserve transcriptional dynamics in embryonic stem cells

Research output: Contribution to journalArticle

Abstract

TRIM28 is critical for the silencing of endogenous retroviruses (ERVs) in embryonic stem (ES) cells. Here, we reveal that an essential impact of this process is the protection of cellular gene expression in early embryos from perturbation by cis-acting activators contained within these retroelements. In TRIM28-depleted ES cells, repressive chromatin marks at ERVs are replaced by histone modifications typical of active enhancers, stimulating transcription of nearby cellular genes, notably those harboring bivalent promoters. Correspondingly, ERV-derived sequences can repress or enhance expression from an adjacent promoter in transgenic embryos depending on their TRIM28 sensitivity in ES cells. TRIM28-mediated control of ERVs is therefore crucial not just to prevent retrotransposition, but more broadly to safeguard the transcriptional dynamics of early embryos.

Details

Authors
  • Helen M. Rowe
  • Adamandia Kapopoulou
  • Andrea Corsinotti
  • Liana Fasching
  • Todd S. Macfarlan
  • Yara Tarabay
  • Stephane Viville
  • Johan Jakobsson
  • Samuel L. Pfaff
  • Didier Trono
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Genetics
Original languageEnglish
Pages (from-to)452-461
JournalGenome Research
Volume23
Issue number3
Publication statusPublished - 2013
Publication categoryResearch
Peer-reviewedYes

Total downloads

No data available

Related research output

Fasching, L., 2015, Molecular Neurogenetics, Faculty of Medicine, Lund University. 74 p.

Research output: ThesisDoctoral Thesis (compilation)

View all (1)