Triolein from Coix lacryma-jobi induces cell cycle arrest through p53/p21 signaling pathway

Research output: Contribution to journalArticle

Abstract

p53, a tumor suppressor protein, has important roles in DNA repair, cell cycle and apoptosis, is a one of the key events in cancer development. Coix lacryma-jobi seed has been used as a food and traditional medicine plant with anti-oxidant, anti-cancer and anti-diabetic effects. In currently research, we identified the most potent p53-increasing compound among 4 compounds (1-4) found in Coix lacryma-jobi and demonstrated its molecular mechanism in MCF-7 cells. Among the four isolated compounds (1-4), triolein most increased p53. Triolein treatment induced p53, p21, p27 and Bax in MCF-7 cells. Moreover, triolein caused S phase arrest through suppression of CDK1, phopho-Rb and E2F1 in dose-dependent manner. We also observed the decreasing of DNA synthesis by triolein. These data suggest that triolein may induced cell cycle restart involve DNA synthesis and apoptosis pathway in MCF-7 cells.

Details

Authors
  • Thi Hien Tran
  • Do Thi Ha
  • Do Minh Truong
  • Loi Vu Duc
  • Trong Tuan Dao
  • Bui Thi Binh
  • Nguyen Thanh Hai
Organisations
External organisations
  • Thai Binh University of Medicine and Pharmacy
  • National Institute of Medicinal Materials, Vietnam
  • Thai Nguyen University
  • Vietnam National University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Pharmaceutical Sciences

Keywords

  • Apoptosis, Coix lacryma-jobi, DNA synthesis, MCF-7 cells, Triolein
Original languageEnglish
Pages (from-to)519-524
Number of pages6
JournalBiomedical and Pharmacology Journal
Volume9
Issue number2
Publication statusPublished - 2016
Publication categoryResearch
Peer-reviewedYes