Turnover of proteoglycans in guinea pig costal cartilage

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Abstract

The turnover in vivo of proteoglycans of guinea pig costal cartilage was investigated using Na2 35SO4 as precursor. Proteoglycans were extracted with guanidine · HCl, at both low and high ionic strength, and purified and fractionated by ultracentrifugation in CsCl gradients under associative and dissociative conditions. The results suggest that the sulfate is incorporated into macromolecules of at least two major metabolic pools with half-lives of about 3 days and about 60-70 days, respectively. Molecules with a fast turnover were enriched in the low ionic strength extracts and in fractions containing small, nonaggregated proteoglycans. No substantial evidence was found for a precursor-product relationship between different fractions.

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Authors
Organisations
External organisations
  • Karolinska Institutet
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Orthopedics
Original languageEnglish
Pages (from-to)93-101
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume180
Issue number1
Publication statusPublished - 1977 Apr 15
Publication categoryResearch
Peer-reviewedYes