Two routes for extracellular electron transfer in Enterococcus faecalis

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Abstract

Enterococcus faecalis cells are known to have ferric reductase activity and the ability to transfer electrons generated in metabolism to the external environment. We have isolated mutants defective in ferric reductase activity and studied their electron transfer properties to electrodes mediated by ferric ions and an osmium complex modified redox polymer (OsRP), respectively. Electron transfer mediated with ferric ions and ferric reductase activity were both found dependent on the membrane associated Ndh3 and EetA proteins, consistent with findings in Listeria monocytogenes. In contrast, electron transfer mediated with OsRP was independent of these two proteins. Quinone in the cell membrane was required for the electron transfer with both mediators. The combined results demonstrate that extracellular electron transfer from reduced quinone to ferric ions and to OsRP occurs via different routes in the cell envelope of E. faecalis.

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Authors
Organisations
External organisations
  • Technical University of Denmark
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology
Original languageEnglish
Article numbere00725-19
Number of pages9
JournalJournal of Bacteriology
Volume202
Issue number7
Publication statusPublished - 2020 Mar 11
Publication categoryResearch
Peer-reviewedYes