Ubiquitous Structural Signaling in Bacterial Phytochromes.
Research output: Contribution to journal › Article
The phytochrome family of light-switchable proteins has long been studied by biochemical, spectroscopic and crystallographic means, while a direct probe for global conformational signal propagation has been lacking. Using solution X-ray scattering, we find that the photosensory cores of several bacterial phytochromes undergo similar large-scale structural changes upon red-light excitation. The data establish that phytochromes with ordinary and inverted photocycles share a structural signaling mechanism and that a particular conserved histidine, previously proposed to be involved in signal propagation, in fact tunes photoresponse.
|Research areas and keywords||
Subject classification (UKÄ) – MANDATORY
|Journal||The Journal of Physical Chemistry Letters|
|Publication status||Published - 2015|