Ultramicrobiosensor for the selective detection of glutamate

Research output: Contribution to journalArticle

Abstract

Carbon fiber microelectrodes, able to detect catecholamine release from single cells, have significantly contributed to our present understanding of the mechanism of secretory neurotransmission. In spite of their obvious advantages, there are only a few amperometric sensors (characterized by appropriate size, sensitivity, and selectivity) able to measure the release of other (not easily oxidizable) neurotransmitters at cellular level. The present work describes the fabrication and characterization of an ultramicrobiosensor for the selective detection of glutamate. ne developed sensor has a size of 2.5 - 15 mu m in diameter, a sensitivity of 0.62 mA mM(-1) cm(-2), and a detection limit of 5 mu M. The excellent selectivity of the sensor (achieved using electrodeposition of Ru, Rh, and poly(m-phenylenediamine)) makes it a promising candidate for monitoring glutamate release at single cell level.

Details

Authors
  • Oleg M. Schuvailo
  • Szilveszter Gaspar
  • Alexey P. Soldatkin
  • Elisabeth Csöregi
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Analytical Chemistry

Keywords

  • interference elimination, glutamate detection, microbiosensor, amperometry, ultramicroelectrode
Original languageEnglish
Pages (from-to)71-78
JournalElectroanalysis
Volume19
Issue number1
Publication statusPublished - 2007
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)