Ultrastructure of alpha-synuclein-positive aggregations in U373 astrocytoma and rat primary glial cells

Research output: Contribution to journalArticle

Abstract

Abnormal alpha-synuclein-positive glial cytoplasmic inclusions are found in Parkinson's disease, multiple system atrophy and dementia with Lewy bodies. We have recently developed an in vitro model of alpha-synuclein-immunoreactive aggregations in U373 astrocytoma cells. We have additionally overexpressed wild-type and a C-terminally truncated form of alpha-synuclein in primary rat glial cells. Astrocytes and oligodendrocytes were found to form alpha-synuclein-positive aggregations in vitro perinuclearly or in the processes of the cells. The morphological studies presented here demonstrate that the aggregations we have observed in vitro are not limited by a membrane but have unclear borders. They have an amorphous dense core that is intensely alpha-synuclein-immunopositive and a predominantly filamentous halo around. Mainly filamentous structures at the border area between the halo and the core are alpha-synuclein-immunoreactive. We conclude that this in vitro model of alpha-synuclein-positive glial aggregations mimics the morphology of the abnormal glial inclusions described in neuroclegenerative disorders and could be a suitable model for studying their role in the pathogenesis of these diseases. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

Details

Authors
  • N Stefanova
  • Mia Emgård-Mattson
  • L Klimaschewski
  • GK Wenning
  • M Reindl
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Neurosciences

Keywords

  • glial cytoplasmic inclusions, ultrastructure, alpha-synuclein, glia, Lewy bodies, neurodegeneration
Original languageEnglish
Pages (from-to)37-40
JournalNeuroscience Letters
Volume323
Issue number1
Publication statusPublished - 2002
Publication categoryResearch
Peer-reviewedYes