Unexpected high genetic diversity in small populations suggests maintenance by associative overdominance

Research output: Contribution to journalArticle

Abstract

textcopyright 2017 John Wiley Sons Ltd. The effective population size (N e ) is a central factor in determining maintenance of genetic variation. The neutral theory predicts that loss of variation depends on N e , with less genetic drift in larger populations. We monitored genetic drift in 42 Drosophila melanogaster populations of different adult census population sizes (10, 50 or 500) using pooled RAD sequencing. In small populations, variation was lost at a substantially lower rate than expected. This observation was consistent across two ecological relevant thermal regimes, one stable and one with a stressful increase in temperature across generations. Estimated ratios between N e and adult census size were consistently higher in small than in larger populations. The finding provides evidence for a slower than expected loss of genetic diversity and consequently a higher than expected long-term evolutionary potential in small fragmented populations. More genetic diversity was retained in areas of low recombination, suggesting that associative overdominance, driven by disfavoured homozygosity of recessive deleterious alleles, is responsible for the maintenance of genetic diversity in smaller populations. Consistent with this hypothesis, the X-chromosome, which is largely free of recessive deleterious alleles due to hemizygosity in males, fits neutral expectations even in small populations. Our experiments provide experimental answers to a range of unexpected patterns in natural populations, ranging from variable diversity on X-chromosomes and autosomes to surprisingly high levels of nucleotide diversity in small populations.

Details

Authors
  • M.F. Schou
  • V. Loeschcke
  • J. Bechsgaard
  • C. Schlötterer
  • T.N. Kristensen
External organisations
  • Aarhus University
  • University of Veterinary Medicine Vienna
  • Aalborg University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Evolutionary Biology

Keywords

  • Effective population size,Evolutionary potential,Genetic diversity,Genetic drift,Nucleotide diversity,Small populations
Original languageEnglish
Pages (from-to)6510-6523
Number of pages14
JournalMolecular Ecology
Volume26
Issue number23
Publication statusPublished - 2017
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes