Using Endogenous MicroRNA Expression Patterns to Visualize Neural Differentiation of Human Pluripotent Stem Cells

Research output: Chapter in Book/Report/Conference proceedingBook chapter

Abstract

Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells.

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Authors
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Neurosciences

Keywords

  • pluripotent stem cells, microRNA
Original languageEnglish
Title of host publicationHuman Embryonic and Induced Pluripotent Stem Cells
Subtitle of host publicationPart of the series Springer Protocols Handbooks
EditorsKaiming Ye, Sha Jin
PublisherSpringer
Pages465-480
ISBN (Electronic)978-1-61779-267-0
ISBN (Print)978-1-61779-266-3
Publication statusPublished - 2011 Jul 30
Publication categoryResearch
Peer-reviewedYes

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