Viral capsids: Mechanical characteristics, genome packaging and delivery mechanisms

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Viral capsids: Mechanical characteristics, genome packaging and delivery mechanisms. / Roos, W. H.; Ivanovska, I. L.; Evilevitch, Alex; Wuite, G. J. L.

In: Cellular and Molecular Life Sciences, Vol. 64, No. 12, 2007, p. 1484-1497.

Research output: Contribution to journalReview article

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Roos, W. H. ; Ivanovska, I. L. ; Evilevitch, Alex ; Wuite, G. J. L. / Viral capsids: Mechanical characteristics, genome packaging and delivery mechanisms. In: Cellular and Molecular Life Sciences. 2007 ; Vol. 64, No. 12. pp. 1484-1497.

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TY - JOUR

T1 - Viral capsids: Mechanical characteristics, genome packaging and delivery mechanisms

AU - Roos, W. H.

AU - Ivanovska, I. L.

AU - Evilevitch, Alex

AU - Wuite, G. J. L.

PY - 2007

Y1 - 2007

N2 - The main functions of viral capsids are to protect, transport and deliver their genome. The mechanical properties of capsids are supposed to be adapted to these tasks. Bacteriophage capsids also need to withstand the high pressures the DNA is exerting onto it as a result of the DNA packaging and its consequent confinement within the capsid. It is proposed that this pressure helps driving the genome into the host, but other mechanisms also seem to play an important role in ejection. DNA packaging and ejection strategies are obviously dependent on the mechanical properties of the capsid. This review focuses on the mechanical properties of viral capsids in general and the elucidation of the biophysical aspects of genome packaging mechanisms and genome delivery processes of double-stranded DNA bacteriophages in particular.

AB - The main functions of viral capsids are to protect, transport and deliver their genome. The mechanical properties of capsids are supposed to be adapted to these tasks. Bacteriophage capsids also need to withstand the high pressures the DNA is exerting onto it as a result of the DNA packaging and its consequent confinement within the capsid. It is proposed that this pressure helps driving the genome into the host, but other mechanisms also seem to play an important role in ejection. DNA packaging and ejection strategies are obviously dependent on the mechanical properties of the capsid. This review focuses on the mechanical properties of viral capsids in general and the elucidation of the biophysical aspects of genome packaging mechanisms and genome delivery processes of double-stranded DNA bacteriophages in particular.

KW - biophysics

KW - optical tweezers

KW - atomic force microscopy

KW - osmotic pressure

KW - genome delivery

KW - capsid mechanics

KW - genome packaging

U2 - 10.1007/s00018-007-6451-1

DO - 10.1007/s00018-007-6451-1

M3 - Review article

VL - 64

SP - 1484

EP - 1497

JO - Cellular and Molecular Life Sciences

JF - Cellular and Molecular Life Sciences

SN - 1420-9071

IS - 12

ER -