Visualisation of ectomycorrhizal rhizomorph structure using laser scanning confocal microscopy

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Abstract

A method for the observation of the three-dimensional structure of intact ectomycorrhizal rhizomorphs is described. The method is based on a combination of clearing the material with KOH followed by staining with congo red and subsequent imaging under a laser scanning confocal microscope (LSCM). The images obtained are of a much higher three-dimensional resolution than those obtained previously by use of conventional light microscopical techniques. The structure of highly differentiated and undifferentiated rhizomorphs is described. Applications of the method are briefly discussed.

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  • Biological Sciences
Original languageEnglish
Pages (from-to)349-354
JournalMycological Research
Volume106
Issue number3
Publication statusPublished - 2002
Publication categoryResearch
Peer-reviewedYes

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