Visualisation of ectomycorrhizal rhizomorph structure using laser scanning confocal microscopy

Research output: Contribution to journalArticle


A method for the observation of the three-dimensional structure of intact ectomycorrhizal rhizomorphs is described. The method is based on a combination of clearing the material with KOH followed by staining with congo red and subsequent imaging under a laser scanning confocal microscope (LSCM). The images obtained are of a much higher three-dimensional resolution than those obtained previously by use of conventional light microscopical techniques. The structure of highly differentiated and undifferentiated rhizomorphs is described. Applications of the method are briefly discussed.


Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biological Sciences
Original languageEnglish
Pages (from-to)349-354
JournalMycological Research
Issue number3
Publication statusPublished - 2002
Publication categoryResearch

Total downloads

No data available