Visualisation of ectomycorrhizal rhizomorph structure using laser scanning confocal microscopy
Research output: Contribution to journal › Article
A method for the observation of the three-dimensional structure of intact ectomycorrhizal rhizomorphs is described. The method is based on a combination of clearing the material with KOH followed by staining with congo red and subsequent imaging under a laser scanning confocal microscope (LSCM). The images obtained are of a much higher three-dimensional resolution than those obtained previously by use of conventional light microscopical techniques. The structure of highly differentiated and undifferentiated rhizomorphs is described. Applications of the method are briefly discussed.
|Research areas and keywords||
Subject classification (UKÄ) – MANDATORY
|Publication status||Published - 2002|
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