Visualization of the melanosome transfer-inhibition in a mouse epidermal cell co-culture model.

Research output: Contribution to journalArticle

Abstract

Transfer of melanin-containing melanosomes from melanocytes to neighboring keratinocytes results in skin pigmentation. To provide a more practical method of visualizing melanosomes in melanocytes as well as in keratinocytes, we attempted to use murine cell lines instead of human primary cells. We generated various fluorescent fusion proteins of tyrosinase, a melanin synthesis enzyme located in the melanosome, by using green fluorescent protein and red fluorescent protein. The intracellular localization of tyrosinase was then examined by fluorescence and confocal microscopy. Co-culture of murine melanocytes and keratinocytes was optimized and melanosome transfer was either stimulated with alphaMSH or partially inhibited by niacinamide. To the best of our knowledge, this is the first study showing that a murine co-culture model, in addition to human primary cell co-culture, can be a good tool for depigmenting agent screening by monitoring melanosome transfer.

Details

Authors
  • Hae Jong Kim
  • Julhash U. Kazi
  • You Ree Lee
  • Dung H. Nguyen
  • Hyang Bok Lee
  • Jeong Hyun Shin
  • Jae Won Soh
  • Eun Ki Kim
External organisations
  • Inha University
Original languageEnglish
Pages (from-to)249-253
JournalInternational Journal of Molecular Medicine
Volume25
Issue number2
Publication statusPublished - 2010 Feb 1
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Experimental Clinical Chemistry (013016010)